Influence of repeated behavioral tests within a short term on cognitive and anxiety assessment in 3xTg-AD mice
10.19845/j.cnki.zfysjjbzz.2025.0139
- VernacularTitle:短期内行为学重复测定对3xTg-AD小鼠认知及焦虑状态评估效果的影响
- Author:
Xiaoyue LI
1
;
Fangzhao LIN
1
;
Haijun CAO
1
Author Information
1. 中国医学科学院北京协和医学院输血研究所，四川 成都 610052
- Publication Type:Journal Article
- Keywords:
Repeated measurement;
3xTg-AD mice;
Cognitive assessment;
Behavioral tests;
Anxiety status
- From:
Journal of Apoplexy and Nervous Diseases
2025;42(8):727-735
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of repeated open field tests， novel object recognition tests， and Barnes maze behavioral tests within a short term on cognitive and anxiety assessment in 3xTg-AD mice.Methods Four groups of 3xTg-AD mice， aged 12 months， were obtained using different pretreatment regimens， and the mice in the experimental groups 1-3 were treated with different drugs， while those in the control group were treated with normal saline. The open field test， the novel object recognition test， and the Barnes maze test were performed on mice successively. A behavioral video analysis system was used to record the locomotor trajectories of the mice and analyze the parameters such as time spent in the central area， exploration time for novel versus familiar objects， and latency to reach the target hole. After one session of complete tests， three sessions were performed repeatedly， and all tests were completed within one month.Results With the increase in the number of repeated tests， there was a significant reduction in the time spent in the central area in the open field test （P<0.05）； in the novel object recognition test， there was an increase in the coefficient of variation for object recognition index and a reduction in exploratory behavior towards both the new environment and the novel objects； in the Barnes maze test， there was no significant difference in the latency to reach the target hole across the four tests， suggesting good reproducibility.Conclusion Repeated tests within a short term can interfere with the evaluation of anxiety status in 3xTg-AD mice in the open field test and their preference for novel objects in the novel object recognition test， but it has no obvious influence on the latency to reach the target hole in the Barnes maze test. Whether behavioral tests can be performed repeatedly in 3xTg-AD mice should be determined based on different tests.
- Full text:2025090413583114382短期内行为学重复测定对3xTg-AD小鼠认知及焦虑状态评估效果的影响.pdf
