Gentic Diversities of Four Short Tandem Repeats Loci in Korean Amplified by Multiplex PCR.
- Author:
Eun Seop SONG
;
Yong Suk NAM
;
Juck Joon HWANG
;
Gil Ro HAN
- Publication Type:Original Article
- MeSH:
Alleles;
Discrimination (Psychology);
DNA;
Gene Frequency;
Genotype;
Microsatellite Repeats*;
Multiplex Polymerase Chain Reaction*;
Paternity
- From:Korean Journal of Legal Medicine
1997;21(1):59-74
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The four tetrameric STRs loci(HUMvWA31, HUMTHO1, HUMF13A1, HUMFES/FPS) were studied to confirm the allele frequency distribution and to see whether these results can be used for identity and paternity testing in a population o Koreans using multiplex PCR and laser-fluorescence detection method. In the Korean population (n=227), 8 alleles with their relative frequency range of 0.002-0.249 are detected in the HUMvWA31 locus, 6 alleles with those of 0.007-0.500 in 6 alleles with those of 0.004-0.434 in the HUMFES/FPS locus. The highest observed heterozygosity is found at the locus HUMvWA31(0.8077), with those of the lociively. All loci meet Hardy-Weinberg expectations ; there are good agreements between observed and expected heterozygosity, number of observed genotypes. Pairwise comparisons between loci show allelic independence for all the 4 loci. The power of discrimination (PD) determined for the locus HUMvWA31 is 0.933, that for the HUMTHO1 is 0.836, 0.798 for HUMF13A1, and 0.844 for the HUMFES/FPS ; the combined power of discrimination for the quadruplex is 0.9997. Thus, these allelic frequency distribution can be used to construct the database of the multiplex PCR-based DNA profile in the Korean population. The calculated parameter, "combined power of discrimination(PD)" show the informativeness of these loci for the determination of identity and relatedness of individuals.
