ANTIOXIDATIVE PROPOLIS FROM HETEROTRIGONA ITAMA AMELIORATES THE EARLY MACROVASCULAR CHANGES IN DIABETIC RATS
https://doi.org/10.22452/jummec.vol25no1.18
- Author:
Boon Seng Yeoh
1
;
Norsuhana Omar
1
;
Siti Nurma Hanim Hadie
1
;
Rozaziana Ahmad
1
;
Onn Zhi Lim
1
;
Aminah Che Romli
1
;
Victor Udo Nna
2
;
Mohaneem Mohamed
1
Author Information
1. Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.
2. Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Nigeria.
- Publication Type:Journal Article
- Keywords:
Stingless bee propolis;
Heterotrigona itama;
antioxidant;
diabetic macroangiopathy;
antiatherogenic effect
- MeSH:
Antioxidants
- From:Journal of University of Malaya Medical Centre
2022;25(1):113-123
- CountryMalaysia
- Language:English
-
Abstract:
Background:Oxidative damage culminates in diabetic macrovascular complication. Ethanolic extract propolis of Heterotrigona itama exhibits antioxidative, antidiabetic and cardioprotective properties. However, vasoprotective activity has yet to be reported. This research was designed to investigate the effects of the ethanolic extract of propolis on histology and oxidative stress parameters in the aorta of diabetic rats.
Methods:Raw propolis from Kelantan bee farm was ground, dissolved and distilled with ethanol into crude extract. Adult male Sprague Dawley rats were divided into four groups, n=8/group: normoglycemia (NG), untreated diabetes (DM), metformin-treated diabetes (DM+M, 300 mg/kg/day) and propolis-treated diabetes (DM+P, 300 mg/kg/day). A single injection of intraperitoneal streptozotocin (60 mg/kg) was used to induce diabetes mellitus, followed by a four-week treatment, via oral gavage, administered immediately after successful diabetes-induction with evidence of hyperglycaemia. Both NG and DM groups were given one millilitre of the vehicle. Rats were sacrificed at the end of the experiment; blood was collected for determination of serum lipid profile; the aorta was fixed and processed for histomorphology and histomorphometry evaluation; aortic tissue homogenate assay for oxidant/antioxidant activities. Differences in the parameters between groups were analysed using one-way analysis of variance (ANOVA) with post-hoc adjustment.
Reults:Propolis treatment elevated the high-density lipoprotein level and suppressed protein oxidation in diabetic rats. Furthermore, propolis halted the thickening of tunica intima and aberrant proliferation of vascular smooth muscle cells in tunica media.
Conclusion:In conclusion, this proof-of-concept study indicated the potential of propolis as a vasoprotective agent against the early pathogenesis of diabetic macroangiopathy through anti-oxidative and anti- hyperglycaemic activities.
- Full text:202508141139388065680286.pdf
