Analysis of metabolites of Semaglutide in human liver microsomes
10.13200/j.cnki.cjb.004545
- VernacularTitle:司美格鲁肽在人肝微粒体中代谢产物的分析
- Author:
LI Ju
- Publication Type:Journal Article
- From:
Chinese Journal of Biologicals
2025;38(08):925-931
- CountryChina
- Language:Chinese
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Abstract:
Objective To analyze the metabolic characteristics and major metabolites of Semaglutide, a glucagon-like peptide-1(GLP-1) receptor agonist, in human liver microsomes.Methods Human liver microsomal incubation system was used to metabolize Semaglutide in vitro. The metabolites were identified by ultra-performance liquid chromatography(UPLC) and X500R quadrupole time-of-flight(QTOF) mass spectrometry.Results Semaglutide underwent extensive metabolism in human liver microsomes, mainly through proteolytic cleavage of peptide skeleton and oxidation of amino acid residues. A total of six metabolites were identified, five of which were discovered for the first time.Conclusion The discovery of Semaglutide metabolites in human liver microsomes provides important information for further study of the pharmacodynamic properties and safety, and contributes to a deeper understanding of the metabolite characteristics of Semaglutide and its implications for clinical application.
