Evaluation of protective effect of two virus protective agents against a novel varicella-herpes zoster virus(Oka-7S)
10.13200/j.cnki.cjb.004169
- VernacularTitle:两种保护剂对新型水痘-带状疱疹病毒（Oka-7S）保护效果评价
- Author:
LI Hang
- Publication Type:Journal Article
- Keywords:
Varicella-herpes zoster virus(VZV);
Oka-7S;
Protective agent;
Freeze-thaw;
Ultrasound;
Temperature;
Virus titer
- From:
Chinese Journal of Biologicals
2025;38(08):908-912+919
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of freeze-thaw, ultrasound and temperature on the novel varicella-herpes zoster virus(VZV)(Oka-7S) under different virus protective agents.Methods Oka-7S virus samples(intracellular and extracellular virus samples) were freeze-thawed for four times under No.1 and No.2 virus protective agents at-80 ℃, and the virus titer was detected at each freeze-thaw. Oka-7S virus samples(intracellular and extracellular virus samples) under different virus protective agents were ultrasonically studied at 20 kHZ for six times, and detected for the virus titer after each ultrasound. The survival time of Oka-7S virus samples(intracellular and extracellular virus samples) under different virus protective agents was detected at 4 ℃, 25 ℃ and 37 ℃.Results Protective agent No.2 had the best protective effect on Oka-7S intracellular and extracellular virus samples. The titer of Oka-7S intracellular virus samples was higher at-80 ℃, and the effect of freeze-thaw once was the best. Under ultrasonic conditions, protective agent No.2 made Oka-7S intracellular virus release more virus particles, and at the same time, Oka-7S extracellular virus samples had higher titer. The Oka-7S intracellular virus samples had the best effect at 20 kHZ for 3 s, while the Oka-7S extracellular virus samples had the best effect at20 kHZ for 1 s. In addition, the survival time of Oka-7S intracellular and extracellular virus samples was longer at 4 ℃, and the preservation time of Oka-7S virus samples was significantly prolonged by protective agent No.2 compared with protective agent No.1.Conclusion Under different preservation conditions, the protective effect of No.2 protective agent on virus was better under freeze-thaw, ultrasound and temperature conditions, and the titer loss of intracellular and extracellular virus samples of Oka-7S was lower.
