Evaluation of uncertainty in the determination of 19 elements in human urine by inductively coupled plasma mass spectrometry
10.3969/j.issn.1006-2483.2025.04.010
- VernacularTitle:电感耦合等离子体质谱法测定人尿中19种元素的不确定度评定
- Author:
Mengxi WU
1
;
Wenyao HUANG
1
;
Ying ZHANG
1
;
Qianqian XIANG
1
;
Ying DENG
1
Author Information
1. Hubei Provincial Center for Disease Control and Prevention ,Wuhan , Hubei 430079 , China
- Publication Type:Journal Article
- Keywords:
Inductively coupled plasma mass spectrometry;
Human urine;
Multi-element;
Uncertainty evaluation
- From:
Journal of Public Health and Preventive Medicine
2025;36(4):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To use direct dilution method to pretreat human urine, and to determine 19 elements in human urine using inductively coupled plasma mass spectrometry, and to evaluate the uncertainty of the entire experimental process. Methods The relevant mathematical models were established according to JJF 10591-2012 “Evaluation and Expression of Measurement Uncertainty” and CNAS-GL006 “Guidance for Evaluation of Uncertainty in Chemical Analysis”. Taking molybdenum as an example, the uncertainty sources in the determination of 19 elements in human urine by inductively coupled plasma method were analyzed and evaluated, including sample pretreatment, sample repeated measurement, standard solution preparation and standard curve fitting. Results The extended uncertainty of molybdenum in human urine is 2.12μg/L, and the measurement result of molybdenum is (44.8±2.12)μg/L. The measurement result of 19 elements in human urine ranges from less than the detection limit to 601μg/L, and the extended uncertainty range is 0.38~33.6μg/L.Conclusion It was found from the calculation that the uncertainty of the determination result was mainly affected by the uncertainty of the sample repeated measurement and the standard curve fitting. By adjusting the range of standard curve and increasing the number of parallel sample measurement, the uncertainty was reduced and the quality of detection was improved.
