Genotyping of M protein gene of group A streptococcus infections among children in Xicheng District of Beijing in 2011-2024
10.3969/j.issn.1006-2483.2025.04.007
- VernacularTitle:2011-2024年北京市西城区儿童A族链球菌感染病例emm基因分型研究
- Author:
Di QIN
1
;
Xiaokan WEI
2
;
Xiaowen PENG
1
;
Xiugang GUAN
1
;
Yanhui CHU
1
Author Information
1. Xicheng District Center for Disease Control and Prevention , Beijing 100120 , China
2. Beijing Center for Disease Control and Prevention , Beijing 100013 , China
- Publication Type:Journal Article
- Keywords:
Children;
Group A streptococcus;
Emm gene;
Scarlet fever
- From:
Journal of Public Health and Preventive Medicine
2025;36(4):32-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the distribution of M protein gene (emm) of group A streptococcus (GAS) infections among children in Xicheng District of Beijing. Methods Throat swab samples from scarlet fever and pharyngeal infection cases were collected in sentinel hospitals of Xicheng District, Beijing from 2011 to 2024. GAS strains were isolated and identified, and emm gene was amplified and sequenced by PCR to determine the genotype. The differences in emm genotype between different groups were compared. Results A total of 3 130 throat swab samples were collected, and 400 GAS strains were isolated, with a positive rate of 12.78%. The highest positive rate was 19.93% in 2011. The positive rate of scarlet fever (45.71%) was higher than that of pharyngeal infection (6.14%) (P<0.001). There were 391 emm gene positive strains, and the differences in the positive rate of emm gene among different cases were statistically significant (P<0.001). A total of 7 genotypes and 27 gene subtypes were detected. Among different groups, the emm genotypes were mainly emm12 and emm1. The emm gene subtypes were mainly emm12.00 and emm1.00. Except for some years, the genotypes and their subtypes were dominated by emm12 and emm12.00, and the distribution differences of the two major genotypes and their subtypes were statistically significant from 2011 to 2019 (P<0.001). There were differences in genotypes and subtypes among different age groups (P=0.002). Conclusion The dominant types of emm genes in group A streptococcus infections among children were emm12 and emm1 in Xicheng District of Beijing from 2011 to 2024, and the dominant gene subtypes were emm12.00 and emm1.00. It is necessary to comprehensively strengthen the monitoring of the epidemic situation and genotype, timely grasp the distribution and variation of emm gene.
