Study on fingerprint establishment，content determination，and spectrum-effect relationship of in vitro antioxidant activity for Bushen ningshen ointment
- VernacularTitle:补肾宁神膏的指纹图谱建立、含量测定及体外抗氧化作用的谱效关系研究
- Author:
Li WANG
1
,
2
;
Wenhua XU
3
;
Yang YANG
4
;
Lingyi XIN
4
;
Zhenyi ZHANG
1
,
2
;
Guangyi YANG
1
Author Information
1. Shenzhen Clinical College of Integrated Chinese and Western Medicine，Guangzhou University of Chinese Medicine，Guangdong Shenzhen 518104，China
2. Dept. of Pharmacy，Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine，Guangdong Shenzhen 518104，China
3. Dept. of Preventive Treatment of Disease，Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine，Guangdong Shenzhen 518104，China
4. Key Laboratory of TCM Clinical Pharmacy，Shenzhen Bao'an Authentic TCM Therapy Hospital，Guangdong Shenzhen 518104，China
- Publication Type:Journal Article
- Keywords:
Bushen ningshen ointment;
fingerprint;
spectrum-effect relationship;
antioxidant activity;
content determination
- From:
China Pharmacy
2025;36(14):1749-1754
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the fingerprint of Bushen ningshen ointment， determine the contents of its major constituents， and investigate its in vitro antioxidant activity. METHODS High performance liquid chromatography （HPLC） fingerprints of 10 batches of Bushen ningshen ointment were established. Similarity evaluation and identification of common peaks were subsequently performed. The contents of 10 components such as salidroside were determined using the same HPLC method. Using the scavenging rates against 2，2′-azino bis（3-ethylbenzothiazoline-6-sulfonic acid） （ABTS） and 1，1-diphenyl-2- picrylhydrazyl （DPPH） radicals， as well as ferric ion reducing antioxidant power （FRAP） as indicators， the anti-oxidant activity of the ointment was evaluated； grey relational analysis and partial least squares regression were conducted using SIMCA 14.1 software to establish the spectrum-effect relationship. RESULTS The fingerprint chromatogram of 10 batches of Bushen ningshen ointment contained 24 common peaks， with similarity values all exceeding 0.96. Eleven peaks were identified as adenosine （peak 1）， salidroside （peak 4）， morroniside （peak 6）， catechin （peak 7）， paeoniflorin （peak 10）， spinosin （peak 11）， ferulic acid （peak 12）， isoquercitrin （peak 13）， E-mail：wli1743@163.com verbascoside （peak 14）， paeonol （peak 23）， and emodin （peak 24）. Content determination results showed that the average contents of salidroside， morroniside， catechin， paeoniflorin， spinosin， ferulic acid， isoquercitrin， verbascoside， paeonol， and emodin were 0.725， 1.962， 0.214， 3.395， 0.124， 0.107， 0.286， 0.019， 0.034 and 0.067 mg/g， respectively. The antioxidant potency composite index （APC） for the 10 batches ranged from 85.08% to 96.35%. Spectrum-effect relationship analysis indicated that all 24 common peaks were positively correlated with the antioxidant capacity. Seventeen peaks had variable importance in projection values ＞1， specitically peaks 2， 5， 6， 7， 9， 10， 13- 21， 23， and 24. CONCLUSIONS This study successfully established the HPLC fingerprint and content determination method for Bushen ningshen ointment. The compounds represented by the 17 common peaks such as morroniside may be the active components contributing to its antioxidant effects.
