Inhibitory Effect of Exosomes Loaded with Ginsenoside Rh2 on Growth of Hepatocellular Carcinoma Cells
10.3971/j.issn.1000-8578.2025.24.1326
- VernacularTitle:装载人参皂苷Rh2的外泌体对肝癌细胞生长的抑制作用
- Author:
Meile LI
1
;
Ziyue ZHAO
2
;
Kai JIN
3
;
Yu’an XIE
3
Author Information
1. Department of Oncology, Jiangbin Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China.
2. Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
3. Reproductive Health and Birth Defects Prevention and Control Key Laboratory of Guangxi Maternity and Child Health Care Hospital, Nanning 530021, China.
- Publication Type:BASICRESEARCH
- Keywords:
Hepatocellular carcinoma;
Ginsenoside rh2;
Exosomes
- From:
Cancer Research on Prevention and Treatment
2025;52(7):578-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of exosomes loaded with ginsenoside Rh2 on the biological functions of hepatocellular carcinoma cells. Methods Both Huh7 and PLC/PRF/5 cell were equally divided into control group, exosome group (Exos group), drug group (G-Rh2 group), and exosomes-loaded-with-ginsenoside Rh2 group (Exos@G-Rh2 group). The effects of each group on the viability, clonogenic ability, migration ability, invasion ability, and apoptotic level of hepatocellular carcinoma cells were detected through CCK-8 assay, colony formation assay, cell scratch assay, Transwell assay, and flow cytometry. Results Compared with the control group, the Exos@G-Rh2 group and G-Rh2 group showed significantly decreased cell viability, clonogenic ability, and migration and invasion capabilities, along with a markedly increased cell apoptosis rate (P<0.05). These changes were more pronounced in the Exos@G-Rh2 group than in the G-Rh2 group (P<0.05). Conclusion Exos@G-Rh2 can effectively inhibit the viability and clonogenic, migration, and invasion abilities of liver cancer cells and induce cell apoptosis. This effect is stronger than that of free G-Rh2 at the same concentration.
