RYBP inhibits bladder cancer cell proliferation and migration by affecting EMT
10.3969/j.issn.1009-8291.2025.06.012
- VernacularTitle:环指蛋白1和阴阳1结合蛋白可能通过影响上皮间质转化途经抑制膀胱癌细胞增殖、迁移
- Author:
Wenyu JIANG
1
;
Renjie ZHANG
1
;
Kaiyu QIAN
1
,
2
;
Xinghuan WANG
1
,
2
Author Information
1. Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan 430071
2. Hubei Key Laboratory of Urological Diseases, Wuhan 430071, China
- Publication Type:Journal Article
- Keywords:
bladder cancer;
RING 1 and YY1 binding protein (RYBP);
polycomb group;
epithelial-mesenchymal transition
- From:
Journal of Modern Urology
2025;30(6):520-526
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of RING 1 and YY1 binding protein (RYBP), a member of the polycomb group (PcG), on bladder cancer cell proliferation and invasion, as well as the underlying mechanisms involved, so as to provide reference for the prevention and treatment of bladder cancer. Methods: Overexpressing and knocking down of RYBP were achieved in bladder cancer cell lines (T24, UM-UC-3, and 5637) via plasmids or siRNAs.Cell proliferation was assessed via thiazolyl blue (MTT) and colony formation assays, whereas migration was evaluated via scratch and Transwell assays.Changes in epithelial-mesenchymal transition (EMT) markers and other related proteins were examined with Western blotting. Results: RYBP overexpression significantly inhibited bladder cancer cell proliferation, invasion, and migration, whereas RYBP knockdown promoted these behaviors.Western blotting results revealed that RYBP overexpression downregulated the expressions of EMT markers N-cadherin, Vimentin, and Slug, but upregulated the expression of E-cadherin.Conversely, RYBP knockdown upregulated the expressions of N-cadherin, Vimentin, and Slug, while reducing the expression of E-cadherin. Conclusion: RYBP appears to inhibit the proliferation and migration of bladder cancer cells via the EMT pathway, indicating its potential application in bladder cancer therapies.
