A preliminary study of mechanosensitive channels Piezo 1 and Piezo 2 promoting neurogenic bladder fibrosis in young rats
10.3969/j.issn.1009-8291.2025.04.014
- VernacularTitle:机械敏感通道Piezo 1和Piezo 2促进幼鼠神经源性膀胱纤维化的初步研究
- Author:
Lei LYU
1
;
Yanping ZHANG
1
;
Qi LI
2
;
Junkui WANG
3
;
Shuai YANG
1
;
Zhaokai ZHOU
1
;
Shuai LI
1
;
Yibo WEN
1
;
Yakai LIU
4
;
Guowei SI
4
;
Xingchen LIU
5
;
Jianguo WEN
1
Author Information
1. Department of Urology, Henan Joint International Paediatric Urodynamic Laboratory, Zhengzhou 450052
2. Department of Pediatric Surgery, Zhengzhou 450052
3. Department of Ultrasound, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052
4. Department of Urology, The First Affiliated Hospital of Xinxiang Medical College, Xinxiang 453000
5. Department of Gynecology, Xinyang Central Hospital, Xinyang 464000, China
- Publication Type:Journal Article
- Keywords:
Piezo 1;
Piezo 2;
neurogenic bladder;
bladder fibrosis;
young rat;
mechanosensitive channel
- From:
Journal of Modern Urology
2025;30(4):343-349
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the changes of mechanosensitive channels Piezos (Piezo 1 and Piezo 2) in neurogenic bladder (NB) of young rats and their effects，so as to provide reference for clinical search of new therapeutic targets. Methods: A total of 30 female young SD rats were divided into 5 groups based on random number table method：sham operation group (sham)，2-week nerve transection group (NB-2W)，6-week nerve transection group (NB-6W)，2-week nerve transection + Piezos inhibitor group (NB-P-2W) and 6-week nerve transection + Piezos inhibitor group (NB-P-6W)，with 6 rats in each group.The NB models were constructed by transecting the L6 and S1 spinal nerves of young rats.The NB-2W and NB-6W groups were not intervened after modeling，while the NB-P-2W and NB-P-6W groups were intraperitoneally injected with Piezos inhibitor GsMTx4 (10 mg/kg) every 2 days after modeling.Bladder cystometry and ultrasound were performed after 2 and 6 weeks of transection.The expressions of Piezos and fibrosis-related indexes (Collagen Ⅰ and α-smooth muscle actin) were detected in bladder tissues. Results: The results of bladder cystometry showed that the basal bladder pressure in NB-2W group was significantly increased，while it was slightly decreased but was still higher in NB-6W group than in the sham group (P<0.05).Basal bladder pressure was lower in NB-P-2W group than in NB-2W group，but was higher than that in the sham group; basal bladder pressure was lower in NB-P-6W group than in NB-6W group，but higher than that in the sham group (P<0.05).Compared with the sham group，the NB-2W and NB-6W groups had firstly increased and then decreased maximum cystometric capacity (MCC) (P<0.05).Compared with NB-2W group，NB-P-2W group had lower bladder leakage point pressure (BLPP)，but higher MCC and bladder compliance (BC) (P<0.05).Compared with NB-6W group，NB-P-6W group had significantly lower BLPP but higher MCC and BC (P<0.05).HE and MASSON staining and ultrasound results showed that，with the extension of nerve transection time，bladder fibrosis gradually worsened，the bladder wall became rough and thickened，calculi were visible inside，and hydronephrosis gradually appeared; the degree of fibrosis in NB-P-2W and NB-P-6W groups was less than that in NB-2W and NB-6W groups，and no hydronephrosis was observed in the upper urinary tract.In addition，Western blotting and immunohistochemical results showed that NB-2W and NB-6W groups had significantly higher relative expression levels of Piezos，Collagen Ⅰ and α-SMA than the sham group (P<0.01)，while NB-P-2W and NB-P-6W groups had lower relative expression levels of Piezos,Collagen Ⅰ and α-SMA than NB-2W and NB-6W groups (P<0.01). Conclusion: The increased expressions of mechanosensitive channels Piezos in NB young rats may be involved in the progression of bladder fibrosis，but its mechanism needs further study.
