Application of non-specific recombinant nucleases in biopharmaceutical industry
10.13200/j.cnki.cjb.004532
- VernacularTitle:非特异性重组核酸酶在生物制药工业中的应用
- Author:
ZHOU Xiaotian
- Publication Type:Journal Article
- Keywords:
Non-specific nucleases(NSNs);
Recombinant enzyme;
Biopharmaceuticals;
Cell and gene therapy;
Vaccine
- From:
Chinese Journal of Biologicals
2025;38(07):850-857+865
- CountryChina
- Language:Chinese
-
Abstract:
Non-specific nucleases(NSNs) are widely distributed in animals, plants, bacteria, fungi, and viruses, and can nonspecifically degrade almost all forms of nucleic acids, including single-stranded, double-stranded, linear, circular, and supercoiled DNA and RNA with no requirement on nucleic acid sequences. NSNs are broadly used in various steps of the biopharmaceutical industry and biotechnology that require the removal of nucleic acids. Currently, only a few non-restrictive nucleases have been studied in detail from both structural and functional aspects. In recent years, in addition to traditional fields such as vaccines, the continuous development of emerging fields such as cell and gene therapy has broadened the scope of application of nucleases on the one hand, but also posed new challenges in terms of reaction temperature, salt concentration, and metal chelator tolerance. This paper summarizes different species of NSNs and analyzes their homology and structural catalytic characteristics. Based on a comprehensive comparison of the basic characteristics of representative NSNs such as Benzonase~?, Denarase~?, and HL-SAN~(TM), this paper summarizes various factors that need to be considered for recombinant nucleases used in the biopharmaceutical industry, and prospects for the future research and development trends of NSNs.
