Effects of fetal bovine serum on quality evaluation of human mesenchymal stem cells
10.13200/j.cnki.cjb.004518
- VernacularTitle:胎牛血清对人间充质干细胞质量评价的影响
- Author:
HAN Xiaoyan
- Publication Type:Journal Article
- Keywords:
Human mesenchymal stem cells(hMSCs);
Fetal bovine serum(FBS);
Immune-regulatory function;
Osteogenic differentiation;
Quality evaluation
- From:
Chinese Journal of Biologicals
2025;38(07):781-789
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore and analyze the effects of different batches of fetal bovine serum(FBS) on the quality evaluation of human mesenchymal stem cells(hMSCs) products and provide reference for the development and quality control of hMSCs products.Methods Retrospective analysis was conducted on the immune-regulatory function data of standard cell line CCRC-hMSC-S1 cultured in medium supplemented with FBS from different batches, based on the quality review data of hMSCs products. The standard cell line CCRC-hMSC-S1 was co-cultured with allogeneic peripheral blood mononuclear cells(PBMCs) from 5-6 donors respectively, and five different batches of FBS(FBS-H, FBS-A, FBS-E1, FBS-E2 and FBS-E3)were added to the co-culture system to clarify the direct effects of different batches of FBS on the detection value of immuneregulatory function by CCRC-hMSC-S1. The hMSCs from eight different individuals were cultured respectively in five medium containing different batches of FBS for two weeks, and the immune-regulatory function and osteogenic differentiation capacity were evaluated to clarify the effects of long-term culture of different batches of FBS on the key quality attributes of hMSCs.Results In the co-culture system of hMSCs and PBMC, the detection value of regulatory T-cell(Treg) promotion rate in FBS-H culture system was significantly lower than that in FBS-A, FBS-E1, FBS-E2 and FBS-E3 culture systems(t = 3. 2,3. 0, 3. 2 and 2. 9, respectively, each P < 0. 05), and the detection value of T lymphocyte proliferation inhibition rate in FBSH culture system was significantly higher than that in FBS-A, FBS-E1, FBS-E2 and FBS-E3 culture systems(t = 3. 7, 3. 4,2. 8 and 3. 0, respectively, each P < 0. 05); the inhibitory ability of hMSCs long-term cultured in FBS-A on Th1 proliferation was significantly lower than that of hMSCs long-term cultured in FBS-H, FBS-E1, FBS-E2 and FBS-E3(t = 3. 0, 2. 4, 3. 7and 3. 3, respectively, each P < 0. 05); the suppressive effect of hMSCs long-term cultured in FBS-H on T lymphocyte proliferation was significantly higher than that of hMSCs long-term cultured in FBS-A, FBS-E1, FBS-E2 and FBS-E3(t = 4. 7,2. 6, 3. 4 and 3. 9, respectively, each P < 0. 05); the alizarin red S staining area of hMSCs long-term cultured in FBS-E3 after osteogenic differentiation was significantly lower than that of hMSCs long-term cultured in FBS-A, FBS-H and FBS-E1(t =4. 1, 5. 5 and 3. 9, respectively, each P < 0. 01).Conclusion In the research and development of hMSCs cell therapy products, it is necessary to pay close attention to the effects of FBS components on the quality evaluation of hMSCs, especially the immune-regulatory function and differentiation potential, and select suitable batches of FBS for the development and quality control of hMSCs products.
