Screening of protective antigens against Trueperella pyogenes infection
10.13200/j.cnki.cjb.004515
- VernacularTitle:预防化脓隐秘杆菌感染保护性抗原的筛选
- Author:
XU Dengfeng
- Publication Type:Journal Article
- Keywords:
Trueperella pyogenes(T.pyogenes);
Subunit vaccine;
Lipoprotein;
Protective antigen
- From:
Chinese Journal of Biologicals
2025;38(07):776-780
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the immune protection rates of the identified antigens and potential antigens against Trueperella pyogenes(T. pyogenes) in order to screen for protective antigens.Methods The ATP binding cassette(ABC)substrate-binding proteins(SBPs) were searched from the proteins annotated in the genome of T.pyogenes strain 2012CQZSH(GenBank: CP012649), and the conservation and strain distribution rate of the genes were analyzed using BLSAT. The screened proteins were expressed in the GST fusion protein expression system, purified by Glutathione-Sepharose 4B and used to immunize 20 female Kunming mice. PBS without recombinant protein was used as control group. On the 14th day after the second immunization, the mice were attacked with lethal doses of T.pyogenes strain ZSH-2020[(8. 9 ± 0. 4) × 10~8CFU]via intraperitoneal injection. The number of mice that died within 21 days after the attack was recorded, and the protein protection rates were calculated. The proteins a with protection rate greater than 80% were judged as protective antigens.Results The genes encoding ALD74485(pyolysin, PLO), ALD74170(iron-binding protein, IBP), ALD74643,ALD73390, ALD73068, ALD73591, and ALD73669 were conserved and widely distributed in the strains, and their recombinant proteins were prepared. The protective rates of recombinant proteins PLO, IBP, and ALD73390 were 87. 5%, 81. 25%,and 81. 25%, respectively, which were higher than the protective rates of other proteins.Conclusion PLO, IBP and ALD-73390 are protective antigens against T.pyogenes infection, and the multi-component vaccine composed of these proteins may enhance the effectiveness of the subunit vaccine against T.pyogenes
