Ameliorative effect of Xuebijing injection on acute lung injury in sepsis by interfering with cGAS/STING pathway
10.11665/j.issn.1000-5048.2024071702
- VernacularTitle:血必净注射液干预cGAS/STING通路对脓毒症急性肺损伤的改善作用
- Author:
Xiangying QIN
1
;
Liyuan ZHANG
;
Jiahu TANG
;
Meng YUE
;
Junping KOU
;
Yuanyuan ZHANG
Author Information
1. 中国药科大学中药学院中药药理与中医药学系, 南京 211198
- Publication Type:Journal Article
- Keywords:
sepsis;
acute lung injury;
Xuebijing injection;
cGAS/STING pathway
- From:
Journal of China Pharmaceutical University
2025;56(3):350-357
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of Xuebijing injection (XBJ) on cGAS/STING pathway in alleviating sepsis-induced acute lung injury (ALI), the mouse sepsis-induced ALI model was established by cecal ligation and puncture (CLP), and the cell inflammation model was constructed by LPS stimulating RAW264.7 cells. The effects of XBJ on lung tissue injury and cGAS/STING pathway-related protein expression in septic mice were investigated by HE staining, ELISA, and Western blot. The results showed that XBJ intervention could alleviate lung tissue injury, reduce serum IL-6, TNF-α, IFN-β, IL-1-β levels, and the expression of cGAS, STING, p-TBK1, and p-IRF3 proteins in lung tissue in vivo, and reduce the mRNA level of related inflammatory factors in RAW264.7 cells and the expression of cGAS/STING pathway proteins in vitro. The results showed that XBJ could play a role in the prevention and treatment of sepsis-induced ALI by inhibiting the inflammatory response via inhibition of the activation of cGAS/STING pathway. This study provides a new molecular mechanism for the clinical prevention and treatment of sepsis-induced acute lung injury with XBJ.
