Detection of CSF hypocretin-1 on the narcolepsy type 1 patients by LC-MS/MS：A primary observation
10.19845/j.cnki.zfysjjbzz.2025.0038
- VernacularTitle:液相层析串联质谱法检测脑脊液食欲素诊断1型发作性睡病的初步探索
- Author:
Xianchao ZHAO
1
;
Yuanyuan TANG
2
;
Changjun SU
1
Author Information
1. 空军军医大学唐都医院神经内科，陕西 西安 710038
2. 广州金域医学检验中心有限公司，广东 广州 510145
- Publication Type:Journal Article
- Keywords:
Narcolepsy;
Hypocretin-1;
Liquid chromatography tandem mass spectrometry;
Radioimmunoassay
- MeSH:
Narcolepsy;
Radioimmunoassay
- From:
Journal of Apoplexy and Nervous Diseases
2025;42(3):195-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective Narcolepsy type 1 （NT1） is known to be associated with low levels of hypocretin-1 （Hcrt-1） in cerebrospinal fluid （CSF）. The standard method for Hcrt-1 measurement is radioimmunoassay （RIA） with imported reagents， but this antibody-dependent method is limited to radiation safety-certified lab， gradual radioactivity degradation， and slow turn-around time. The purpose of this study is to explore a non-radioactive， faster， and antibody independent domestic method in China for Hcrt-1 detection. Methods Repeated testing of cerebrospinal fluid from 14 clinically diagnosed NT1 patients and 10 non-narcolepsy patients was performed using liquid chromatography-tandem mass spectrometry （LC-MS/MS）technology，including the establishment and optimization of fundamental methodological procedures. The main steps involved the addition of non-radioactive isotope-labeled internal standards to the cerebrospinal fluid， followed by solid-phase extraction， mass spectrometry signal acquisition， and quantitative analysis. The results were then compared with the corresponding radioimmunoassay（RIA） findings. Results The LC-MS/MS method showed faster speed， and good linearity across a wider range of synthesized standard（5~2 500 pg/ml）， and good repeatability. Although this absolute-quantitation-based LC-MS/MS method and RIA method have different reading values in Hcrt-1 quantitation， they both can segregate NT1 group from non-NT1 group well. Conclusion Although larger cohorts are needed to set up a standard method in China，LC-MS/MS method is proved to be an easier， safer， faster， and possibly more accurate method for Hcrt-1 quantitation and detection for NT1 diagnosis.
- Full text:2025071608472869216液相层析串联质谱法检测脑脊液食欲素诊断1型发作性睡病的初步探索.pdf
