Identification of chemical components and determination of vitexin in the raw powder of Tongluo Shenggu capsule
10.11665/j.issn.1000-5048.2024090301
- VernacularTitle:通络生骨胶囊原粉化学成分鉴定及牡荆苷含量测定
- Author:
Gelin WU
1
,
2
,
3
,
4
;
Ruixin FAN
;
Chuling LIANG
;
Leng XING
;
Yongjian XIE
;
Ping GONG
;
Peng ZHOU
;
BO LI
Author Information
1. 浙江赞生药业有限公司, 台州318000
2. 杭州赞生医药有限公司, 杭州310052
3. 中国药科大学(杭州)创新药物研究院, 杭州310018
4. 中国药科大学药学院, 南京211198 详细信息
- Publication Type:Journal Article
- Keywords:
Tongluo Shenggu capsule;
chemical components;
UPLC-MS/MS;
vitexin;
HPLC
- From:
Journal of China Pharmaceutical University
2025;56(2):166-175
- CountryChina
- Language:Chinese
-
Abstract:
The present study employed UPLC-MS/MS to analyze and identify compounds in the raw powder of Tongluo Shenggu capsules. An HPLC method for the determination of vitexin content was established. The analysis of this drug was performed on a 30 ℃ thermostatic Acquity UPLC® BEH C18 (2.1 mm×100 mm,1.7 μm) column, with the mobile phase comprising 0.2% formic acid-methanol flowing at 0.3 mL /min in a gradient elution manner. Mass spectrometry was detected by ESI sources in both positive and negative ion modes for qualitative identification of chemical constituents. 12 flavonoid and 3 stilbenes compounds in the raw powder of Tongluo Shenggu capsules were successfully identified. Additionally, an HPLC method for the determination of vitexin content was established using a XBridge C18 column (4.6 mm × 250 mm, 5 µm) with a mobile phase of 0.05% glacial acetic acid in methanol for gradient elution, at a column temperature of 30 °C, a flow rate of 1.0 mL/min, and an injection volume of 20 μL. The method demonstrated good linearity in the concentration range of 10 µg/mL to 40 µg/mL (R=1.000) with an average recovery rate of 96.7%. The establishment of these methods provides a scientific basis for the quality control and development of the raw powder of Tongluo Shenggu capsules.
