Differentially expressed proteins in interosseous muscle tissue between patients with familial amyotrophic lateral sclerosis and normal individuals in a family
10.19845/j.cnki.zfysjjbzz.2025.0001
- VernacularTitle:同一家系ALS患者和正常人骨间肌组织的差异蛋白分析
- Author:
Ling LI
1
,
2
;
Yinlan HUANG
2
Author Information
1. 宁夏回族自治区人民医院，宁夏 银川 750001
2. 宁夏医科大学，宁夏 银川 750004
- Publication Type:Journal Article
- Keywords:
Familial amyotrophic lateral sclerosis;
Proteomics;
Isobaric tags for relative and absolute quantitation technique
- MeSH:
Proteomics
- From:
Journal of Apoplexy and Nervous Diseases
2025;42(1):3-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differential expression of related proteins in interosseous muscle tissue between patients with familial amyotrophic lateral sclerosis（FALS） and normal individuals in a family using the isobaric tags for relative and absolute quantitation （iTRAQ） technique， to identify the pathogenic proteins for this family， and to provide a basis for treatment. Methods Interosseous muscle tissue samples were collected from all subjects in this family， and the iTRAQ technique was used to perform qualitative and quantitative analyses for all proteins and obtain the expression profile of proteins in the disease group and the normal group. The bioinformatics methods were used to identify the proteins associated with the onset of FALS. A gene ontology（GO）analysis was performed for cell components， and a classification analysis was performed for related proteins. Results A total of 453 proteins were identified by mass spectrometry. The GO analysis obtained 14 differentially expressed proteins between the disease group and the normal group （P<0.05）， and compared with the normal group，the disease group had the low expression of 5 proteins （Ratio<1） and the high expression of 9 proteins （Ratio>1）. Conclusion This study identifies 8 proteins that are highly associated with FALS， i.e.， tripartite motif-containing protein 72， NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1， annexin A1， decorin， glutathione peroxidase 3， collagen alpha-1 （Ⅻ） chain， collagen alpha-2 （Ⅰ） chain， and collagen type I alpha 1 isoform CRA-a. There are 6 proteins that might be associated with FALS， i.e.，26 S protease regulatory subunit 8， laminin subunit alpha-2，prolargin， fibrillin-1， myosin-8， and dermatopontin.
- Full text:2025071508541406043同一家系ALS患者和正常人骨间肌组织的差异蛋白分析.pdf
