Therapeutic effect and mechanism of hordenine on ovalbumin-induced allergic rhinitis in rats
10.11665/j.issn.1000-5048.2024032802
- VernacularTitle:大麦芽碱对卵清蛋白诱导的大鼠变应性鼻炎的治疗作用及机制
- Author:
Junyan LI
1
,
2
,
3
,
4
,
5
;
Tao LIU
;
Fang SUN
;
Jiahui HUANG
;
Shuzhen MAO
;
Jing YAO
Author Information
1. 山东第二医科大学基础医学院临床病理系, 潍坊 261053
2. 济宁医学院附属医院耳鼻喉头颈外科, 济宁 272067
3. 济宁医学院基础医学院, 济宁 272067
4. 山东第一医科大学药学院, 济南 250117
5. 济宁市药理学重点实验室, 济宁 272067
- Publication Type:Journal Article
- Keywords:
allergic rhinitis;
hordenine;
PI3K/Akt pathway;
Th1/Th2 cell balance
- From:
Journal of China Pharmaceutical University
2025;56(1):80-90
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the therapeutic effect and related mechanisms of hordenine on ovalbumin (OVA)-induced allergic rhinitis (AR) in rats, HE and AB-PAS staining were used to detect the improvement of pathological damage to the nasal mucosa induced by hordenine. ELISA was employed to detect the effect of hordenine on OVA-sIgE in serum and IL-4 in the nasal mucosa supernatant of rats. IHC and Western blot experiments were undertaken to examine the effect of hordenine on Th1/Th2 cell balance. Bioinformatics analysis was performed to predict pathways, which were verified by in vivo and in vitro experiments. The experimental results showed that hordenine could alleviate the behavioral manifestations of OVA-induced AR rats, alleviate nasal mucosal pathological damage caused by AR, and reduce the secretion of OVA-sIgE and IL-4. In addition, hordenine could regulate the Th1/Th2 balance. Bioinformatics analysis results showed that the potential pathway of action of hordenine on AR was the phosphoinositide 3 kinase (PI3K)/protein kinase B (Akt) signaling pathway. The in vivo experimental results showed that the expression of PI3K and p-Akt proteins in the nasal mucosa of the model group rats was significantly increased (P < 0.01), and that the protein expression level was significantly decreased after the administration of hordenine, which was also confirmed by an in vitro experiment. This study suggests that hordenine may regulate Th1/Th2 cell balance through the PI3K/Akt signaling pathway, thereby exerting an alleviating effect on OVA-induced AR.
