Effect of aconite decoction on the activity and polarization of mouse RAW264.7 macrophages
10.12206/j.issn.2097-2024.202505043
- VernacularTitle:乌头煎剂对小鼠RAW264.7巨噬细胞活性和极化的影响
- Author:
Mingcong SHAO
1
;
Hubo CHEN
1
;
Yidan ZHANG
1
;
Ziyan LI
1
;
Lina WANG
1
Author Information
1. Department of Traditional Chinese Medicine, Naval Medical University, Shanghai 200433, China.
- Publication Type:Originalarticles
- Keywords:
aconite decoction;
macrophage polarization;
M1/M2 phenotype;
immunotherapy
- From:
Journal of Pharmaceutical Practice and Service
2025;43(7):329-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Aconite decoction （AD） on the viability and polarization of murine RAW264.7 macrophages induced by lipopolysaccharide （LPS） or interleukin-4 （IL-4）. Methods Cytotoxicity of AD was assessed by the CCK-8 assay. RAW264.7 cells were polarized toward M1 phenotype by LPS or M2 phenotype by IL-4, followed by treatment with varying concentrations of AD. Macrophage polarization was analyzed by flow cytometry. Quantitative PCR was performed to measure mRNA expression of polarization-associated markers （IL-6, iNOS, Arg1, and Ym1）. ELISA was used to quantify secreted cytokines （TNF-α and IL-10）in the supernatant. Results At non-toxic concentrations, IL-6 and iNOS mRNA levels in LPS-stimulated cells were significantly upregulated while Arg1 and Ym1 expression in IL-4-treated groups were downregulated by AD. Concurrently, TNF-α secretion in LPS-induced M1 polarization was enhanced but IL-10 production in IL-4-induced M2 polarization was suppressed by AD. Conclusion AD could promote macrophage proliferation and viability, augments LPS-driven M1 polarization, and inhibit IL-4-mediated M2 polarization, which provided experimental evidence for the potential application of AD in tumor immunotherapy.
