Simultaneous determination of 13 aromatic amine compounds in workplace air by high performance liquid chromatography
10.20001/j.issn.2095-2619.20250411
- VernacularTitle:高效液相色谱法同时测定工作场所空气中13种芳香族胺类化合物
- Author:
Weimin XIE
1
;
Ruibo MENG
;
Zuofei XIE
;
Jing YUAN
;
Jiaheng HE
;
Jiawen HU
;
Weifeng RONG
Author Information
1. School of Public Health, Guangzhou Medical University, Guangzhou, Guangdong 511436, China
- Publication Type:Journal Article
- Keywords:
Aromatic amine compounds;
High performance liquid chromatography;
Workplace;
Air;
Detection method
- From:
China Occupational Medicine
2025;52(2):182-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a liquid chromatography method for the simultaneous determination of 13 aromatic amine compounds (AAs) in workplace air. Methods A total of 13 AAs in both vapor and aerosol phases were collected in workplace air using a new GDH-6 sampling tube. Samples were desorbed and eluted with methanol, separated using a Symmetry Shield™ RP18 reversed-phase liquid chromatography column, and detected with a diode array detector. Quantification was performed using an external standard method. Results The linear range of the 13 AAs measured by this method was 0.02-373.60 μg/L with the correlation coefficients greater than 0.999 0. The minimum detection concentration was 0.09-14.37 μg/m3, and the minimum quantitative concentration was 0.31-47.90 μg/m3 (both calculated based on sampling 15.0 L of air and 3.0 mL of elution volume). The average desorption and elution efficiency ranged from 97.46% to 101.23%. The within-run relative standard deviation (RSD) was 0.10%-5.99%, and the between-run RSD was 0.17%-2.71%. Samples could be stably stored in sealed conditions at 2-8 ℃ for more than seven days. Conclusion This method is suitable for the simultaneous determination of 13 AAs in workplace air, including both vapor and aerosol phases.
