Differential Analysis on Chemical Composition and Pharmacodynamic Effect Between Combined Decoction and Single Decoction of Famous Classical Formula Huaganjian
10.13422/j.cnki.syfjx.20251261
- VernacularTitle:经典名方化肝煎复方合煎与单煎化学成分和药效作用的差异性分析
- Author:
Yang WANG
1
;
Gaoju ZHANG
2
;
Ling LI
1
;
Liping CHEN
1
;
Li ZHANG
1
;
Xiao LIU
1
;
Yuyu ZHANG
1
;
Yuan CUI
1
;
Minglong LI
1
;
Chaomei FU
3
;
Xin YAN
4
;
Yuxin HE
1
;
Qin DONG
1
Author Information
1. Food Microbiology Key Laboratory of Sichuan Province，School of Food Science and Biological Engineering，Xihua University，Chengdu 610039，China
2. Sichuan Traditional Chinese Medicine Decoction Co. Ltd.，Chengdu 611732，China
3. State Key Laboratory of Southwestern Chinese Medicine Resources， School of Pharmacy，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China
4. Chengdu Institute of Chinese Herbal Medicine，Chengdu 610016，China
- Publication Type:Journal Article
- Keywords:
Huaganjian;
famous classical formulas;
combined decoction;
single decoction;
ultra performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry（UPLC-Q-Exactive Orbitrap MS）;
determination;
nonalcoholic fatty liver disease （NAFLD）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(16):199-207
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThrough qualitatively and quantitatively analysis of the differences in chemical composition between the combined decoction and single decoction of Huaganjian and comparison of their core efficacy， to explore the rationality of the flexible clinical application of Huaganjian compound preparations and single-flavored dispensing granules. MethodsUltra performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry（UPLC-Q-Exactive Orbitrap MS） was used to qualitatively analyze the combined decoction and single decoction samples of Huaganjian， and meanwhile， the contents of four index components（geniposide， paeoniflorin， hesperidin and paeonol） were quantitatively analyzed by high performance liquid chromatography（HPLC）. Nonalcoholic fatty liver disease（NAFLD） rat model induced by high-fat diet was applied to compare the efficacy of combined decoction and single decoction of Huaganjian. A total of 30 male SD rats were randomly divided into the control group， model group， lovastatin group（1.8 mg·kg-1）， combined decoction group（1.26 g·kg-1） and single decoction group（1.18 g·kg-1）. After successful modeling， lovastatin group， combined decoction group and single decoction group were given corresponding doses of drugs by intragastric administration every day， and the control group and model group were given equal amounts of normal saline by intragastric administration， after 4 weeks of administration， the serum and liver tissues were collected， and the contents of alanine aminotransferase（ALT）， aspartate aminotransferase（AST）， total cholesterol（TC）， triglyceride（TG）， low-density lipoprotein cholesterol（LDL-C） and high-density lipoprotein cholesterol（HDL-C） in serum of rats were detected， and the liver pathological examination was carried out by hematoxylin-eosin（HE） staining and oil red O staining， so as to compare differences of their efficacy. ResultsSeventy chemical components were initially identified and attributed from the lyophilized powder of the combined decoction and single decoction samples of Huaganjian， and there was no obvious difference in composition between the two. Further quantitative analysis showed that the contents of geniposide， paeoniflorin， hesperidin and paeonol in the combined decoction samples were significantly increased when compared with those of the single decoction samples（P<0.01）. The pharmacodynamic results showed that compared with the model group， both the combined and single decoction groups of Huaganjian could improve the liver index of NAFLD rats， reduce the serum levels of AST， ALT， TC， TG and LDL-C， increase the serum level of HDL-C， and ameliorate the pathological changes of liver cell steatosis and fat accumulation. However， there was no significant difference in pharmacodynamic effects between the combined decoction group and the single decoction group. ConclusionThere is no significant difference between the combined decoction and single decoction of Huaganjian in terms of chemical composition， but the contents of the four index components show significantly difference. Both of them can significantly improve the fat accumulation and liver function in NAFLD rats. This study provides a reference basis for the rational clinical application and evaluation of famous classical formula compound preparations and single-flavored dispensing granules.
