Effects of anesthesia and surgery on the expression of Alzheimer’s disease-related proteins in the hippocampus of 5×FAD mice and its sex differences
10.12025/j.issn.1008-6358.2025.20241401
- VernacularTitle:麻醉和手术对5×FAD小鼠海马内阿尔茨海默病相关蛋白表达影响及性别差异
- Author:
Yinglin ZHANG
1
;
Yong HUANG
1
;
Li ZHANG
1
;
Chao LIANG
1
Author Information
1. Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
- Publication Type:Shortarticle
- Keywords:
Alzheimer’s disease;
anesthesia;
surgery;
5×FAD transgenic mice;
sex differences;
β-catenin/GSK3β
- From:
Chinese Journal of Clinical Medicine
2025;32(3):493-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of anesthesia and surgery on hippocampal expression of Alzheimer’s disease (AD)-associated proteins in 5×FAD transgenic mice and explore potential sex differences. Methods 5×FAD mice were crossbred with C57BL/6J wild-type (WT) mice to generate offspring for genotypic confirmation. Four-month-old 5×FAD mice and littermate (LM) WT controls were allocated into 8 experimental groups (n＝8/group): female/male 5×FAD control group, female/male 5×FAD anesthesia/surgery group, female/male LM control group, and female/male LM anesthesia/surgery group. Anesthesia/surgery groups underwent laparotomy under 1.4% isoflurane anesthesia, while control groups received no intervention. Hippocampal tissues were collected 24 hours post-procedure for Western blotting analysis of β-catenin, glycogen synthase kinase 3 beta (GSK3β), and phosphorylated GSK3β (p-GSK3β) levels. Results Female 5×FAD mice demonstrated significant reductions in β-catenin levels and p-GSK3β expression compared to both sex-matched LM controls and male 5×FAD counterparts (P＜0.05). No significant differences in these proteins were observed in male 5×FAD mice following anesthesia/surgery. Conclusions These findings reveal sex-specific responses to perioperative stress in AD, suggesting that anesthesia and surgery may affect female AD patients through hippocampal β-catenin/GSK3β pathway modulation.
