Impact of dairy farming on gut microbiota structure and diversity of practitioners

Zhaojie WANG; Xixiao MA; Xianxia LIU; Yanggui CHEN; Xueying XIANG; Wanting XU; Jiguo JIN; Fan WU; Xiangnan WEI; Jianyong WU; Fuye LI

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Impact of dairy farming on gut microbiota structure and diversity of practitioners

VernacularTitle:奶牛养殖对从业人员肠道菌群结构及多样性的影响
Author: Zhaojie WANG ¹ ; Xixiao MA ¹ ; Xianxia LIU ² ; Yanggui CHEN ³ ; Xueying XIANG ¹ ; Wanting XU ¹ ; Jiguo JIN ¹ ; Fan WU ¹ ; Xiangnan WEI ¹ ; Jianyong WU ¹ ; Fuye LI ¹
Author Information

1. Institute of Medical Sciences of Xinjiang Medical University, School of Public Health, Xinjiang Medical University, Urumqi, Xinjiang 830017, China.
2. College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China.
3. Urumqi Center for Disease Control and Prevention, Urumqi, Xinjiang 830052, China.
Publication Type:Selectedarticle
Keywords: occupational exposure; dairy farming; 16S rRNA; gut microbiota; microbiota structure; microbiota diversity
From: Journal of Environmental and Occupational Medicine 2025;42(6):668-673
CountryChina
Language:Chinese
Abstract: Background Animal farming may affect the structure and diversity of gut microbiota of farm workers, but it needs more studies to provide solid evidence. Objective To analyze the diversity characteristics of gut microbiota in dairy farm workers, dairy cows, and the control population (non-animal contact occupational group), and to assess the impact of dairy farming on the gut microbiota of workers. Methods The 16S rRNA full-length amplicon sequencing technology was used to sequence 60 fecal samples from dairy farm workers, 89 from dairy cows, and 50 from the general population. The gut microbiota structure characteristics, including operational taxonomic units (OTUs), alpha diversity, beta diversity, and the composition of species at the phylum, family, and genus levels were analyzed. The differences in gut microbiota among the three groups of samples were compared to explore the impact of occupational exposure on the gut microbiota structure of dairy farm workers. Results A total of 50478 OTUs were identified in the gut microbiota of farm workers, dairy cows, and the general population, with 23613, 42723, and 16592 OTUs, respectively. The number of shared OTUs between the dairy cows and the general population was 12674 (27.17%), between the general population and the farm workers was 10099 (33.54%), and between the dairy cows and the farm workers was 17690 (36.36%). Compared with the general population, the farm workers and dairy cows showed more shared gut microbiota (P<0.01). The abundance of Firmicutes in the gut of the farm workers was higher than that of the general population (P<0.01), while Actinobacteriota and Proteobacteria were the opposite (P<0.01). The alpha diversity of gut microbiota in the farm workers was different from that of the general population. The ACE index of the farm workers was slightly lower than that of the general population and much lower than that of dairy cows (P<0.05), while their Shannon index was higher than that of the general population but lower than that of dairy cows (P<0.01). The linear discriminant analysis effect size (LefSe) showed that the abundances of Lachnospiraceae and Ruminococcaceae in the gut microbiota of the farm workers were significantly higher than those of the general population, while Streptococcaceae, Lactobacillaceae, Bifidobacteriaceae, Enterobacteriaceae, and Erysipelatoclostridiaceae were lower than those of the general population. Conclusion The dairy farm workers shares a certain proportion of common bacterial communities with dairy cows. Dairy farming operations can affect the structure and diversity of the gut microbiota of workers by increasing the abundances of Lachnospiraceae and Ruminococcaceae, while decreasing the abundances of Streptococcaceae, Lactobacillaceae, Bifidobacteriaceae, Enterobacteriaceae, and Erysipelatoclostridiaceae. The study results provide reference data for further exploring the cross-host transmission of pathogens related to occupational exposure in dairy farming.