Application and analysis of compatible platelets matching via antigen avoidance
10.13303/j.cjbt.issn.1004-549x.2025.06.015
- VernacularTitle:规避抗体对应抗原模式搜寻配合血小板的应用与分析
- Author:
Shaoyun WU
1
;
Jianxun ZHENG
1
;
Sujun JIANG
1
;
Shiquan WU
1
Author Information
1. Quzhou Central Blood Station, Quzhou 324003, China
- Publication Type:Journal Article
- Keywords:
immune-mediated platelet transfusion refractoriness;
platelet gene bank;
HLA antibodies;
compatible platelet
- From:
Chinese Journal of Blood Transfusion
2025;38(6):839-842
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the application of antigen avoidance pattern for compatible platelets matching. Methods: Samples from two patients with immune-mediated platelet transfusion refractoriness were screened for platelet antibodies using solid-phase red blood cell adhesion assay (SPRCA). The genotypes of HLA-A, -B loci were determined via ploymerase chain reaction sequence. The specificity of HLA class I antibodies was detected using Luminex technology. Results: Platelet antibody screening via SPRCA yielded positive results in both samples. Antibody specificity testing showed the presence of antibodies against HLA-B65, A80, B13, as well as antibodies against HLA-A11, B52, A24 respectively, with both patients exhibiting 80 kinds of positive antibodies. The antibody avoidance pattern successfully selected compatible platelets for transfusion. The bleeding symptoms of two patients were improved after compatible platelets transfusion. Conclusion: For blood stations with limited platelet gene bank resources, antibody avoidance pattern for compatible platelets matching represents an effective strategy for immune-mediated platelet transfusion refractoriness.
