Effect of storage conditions on long-term preservation of PRP growth factors
10.13303/j.cjbt.issn.1004-549x.2025.06.002
- VernacularTitle:储存条件对长期保存PRP生长因子的影响
- Author:
Qing QI
1
;
Zhaojie LI
1
;
Qiong WU
2
;
Pingping MAO
1
;
Yangzi SUN
1
;
Jianfeng LUAN
1
;
Shujun WANG
1
Author Information
1. Department of Blood Transfusion, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China
2. Department of Scientific Research and Training, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China
- Publication Type:Journal Article
- Keywords:
platelet-rich plasma;
growth factor;
storage time;
frozen;
freeze-dried
- From:
Chinese Journal of Blood Transfusion
2025;38(6):759-765
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the changes in the concentration of relevant growth factors released from platelet-rich plasma (PRP) stored at -80℃ by cryopreservation and at 4℃ by refrigerated lyophilization over 2 years, aiming to provide a theoretical basis for prolonging PRP storage duration. Methods: PRP (n=15) was separated using a blood cell separator and stored under -80℃ cryopreservation (F-PRP group) and 4℃ refrigerated freeze-drying conditions (FD-PRP group). The contents of growth factors (PDGF-AA, PDGF-BB, EGF, TGF-β1, and VEGF) in both groups were measured by ELISA at 1, 3, 6, 9, 12 and 24 months. Results: PDGF-AA and VEGF maintained good stability in both groups for up to 24 months. PDGF-BB and TGF-β1 showed high stability in the first 12 months but their stability decreased gradually from 12th to 24th months. EGF demonstrated good stability in the first 6 months, and its stability gradually decreased from the 9th to 24th months. Comparing the F-PRP and FD-PRP groups, the concentrations of the five growth factors in the FD-PRP group were either not statistically different or higher than those in the F-PRP group at all time points. Specifically, the concentrations of EGF were significantly higher in the FD-PRP group at all time points. Conclusion: Both -80℃ freezing and 4℃ freeze-drying enable long-term preservation of PRP. Freeze-drying imposes less stringent storage requirements and facilitates growth factor compared to frozen storage.
