Intervention of Sinomenine in Rat Osteoarthritis by Modulating FABP4
10.13422/j.cnki.syfjx.20242338
- VernacularTitle:青藤碱通过调控FABP4干预大鼠骨关节炎
- Author:
Shiqi ZHANG
1
;
Jun ZHAO
2
;
Ningning LIU
2
;
Yu ZHANG
2
;
Yifei ZHAO
2
;
Jiao LIU
1
;
Hongjian YU
2
Author Information
1. Hebei University of Chinese Medicine， Shijiazhuang 050200， China
2. Binzhou People's Hospital， Binzhou 256610， China
- Publication Type:Journal Article
- Keywords:
sinomenine;
osteoarthritis;
obesity;
synovial tissue;
fatty acid binding protein 4 （FABP4）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(15):115-123
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThis study aims to observe the effect of sinomenine （SIN） on fatty acid binding protein 4 （FABP4） in synovial tissue of rats with osteoarthritis （OA） and investigate the therapeutic mechanism of SIN on OA， further providing new ideas for the management of osteoarthritis by traditional Chinese medicine （TCM）. MethodsAn OA rat model was constructed and randomly divided into a control group， an OA group， an OA + SIN-L group （50 mg·kg-1）， an OA + SIN-M （100 mg·kg-1）， an OA + SIN-H （200 mg·kg-1）， and an OA + prednisone （PDN） group （5 mg·kg-1）. Following surgical modeling for three weeks， an appropriate medication was administered for four weeks. During modeling and administration， a hot plate test was performed to detect the pain and swelling of the knee joints of the rats. The periarticular tissue was collected for arthropathological observation at the end of drug administration. The expression of cleaved Caspase-3， B-cell lymphoma-2 （Bcl-2）， Bcl-2 associated X protein （Bax）， and FABP4 in the synovial tissue of rats was detected by Western blot and real-time quantitative polymerase chain reaction （Real-time PCR）， and the expression and distribution of FABP4 protein in the synovial membrane were detected by immunofluorescence. ResultsCompared with those in the control group， the levels of inflammatory factors and FABP4 in the serum of rats in the OA group were significantly increased （P<0.05， P<0.01）， and joint swelling was significantly elevated （P<0.01）. The thermal pain threshold was significantly reduced （P<0.01）， and the expression of FABP4 protein and the fluorescence intensity were significantly increased （P<0.01）. The synovial tissue exhibited significantly increased inflammatory infiltration， proliferated fibroblasts， and an elevated apoptotic index （P<0.05， P<0.01）. Compared with those in the OA group， the serum lipid metabolism indexes of rats in the SIN administration group gradually returned to normal （P<0.05， P<0.01）， while the levels of inflammatory factors and FABP4 in the serum of rats in the SIN-administered group were significantly reduced （P<0.05， P<0.01）， and joint swelling was significantly decreased （P<0.05， P<0.01）. The thermal pain threshold was significantly elevated （P<0.05， P<0.01）， and the expression of FABP4 protein and fluorescence intensity in the synovial tissue were significantly decreased （P<0.05， P<0.01）. The synovial tissue displayed significantly reduced inflammatory infiltration and a decreased apoptotic index （P<0.05， P<0.01）. ConclusionThe therapeutic effect of SIN on OA may be related to the down-regulation of FABP4 expression， reduction of apoptosis， and inhibition of inflammatory factor expression.
