Effect of Shenshu Fujian Decoction on PDGF/NKD2/Wnt Signaling Pathway in Rats with Chronic Renal Failure
10.13422/j.cnki.syfjx.20242239
- VernacularTitle:肾舒复健汤对慢性肾衰竭大鼠PDGF/NKD2/Wnt信号通路的影响
- Author:
Peng DENG
1
;
Xuekuan HUANG
1
;
Hongyu LUO
1
;
Yuxia JIN
2
;
Dandan WANG
1
;
Xin CHEN
1
;
Shuxian YANG
1
;
Honglin WANG
1
;
Munan WANG
1
Author Information
1. College of Traditional Chinese Medicine， Chongqing Medical University， Chongqing 400016， China
2. Chongqing University of Chinese Medicine， Chongqing 402760， China
- Publication Type:Journal Article
- Keywords:
Shenshu Fujian decoction;
chronic renal failure;
myofibroblast;
platelet-derived growth factor （PDGF）/naked cuticle homolog 2 （NKD2）/Wnt signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(15):79-86
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of Shenshu Fujian decoction on platelet-derived growth factor （PDGF）/naked cuticle homolog 2 （NKD2） /Wnt signaling pathway in rats with chronic renal failure （CRF）. MethodsSixty male SD rats were randomly divided into normal group， model group， Niaoduqing group （5 g·kg-1）， low-dose Shenshu Fujian decoction group （5.5 g·kg-1）， medium-dose Shenshu Fujian decoction group （11 g·kg-1）， and high-dose Shenshu Fujian decoction group （22 g·kg-1）， with 10 rats in each group. A CRF rat model was established by feeding a 0.5% adenine diet for 21 days. After successful modeling， intragastric administration was given once daily for 28 consecutive days. After treatment， the renal morphology of rats was observed. Serum creatinine （SCr） and blood urea nitrogen （BUN） levels were detected. Hematoxylin-eosin （HE） staining and Masson staining were used to detect renal histopathological changes， and collagen volume fraction （CVF） was calculated. Serum levels of inflammatory markers interleukin （IL）-1β and IL-6 were measured using enzyme-linked immunosorbent assay （ELISA）. The expressions of fibronectin 1 （FN1）， type Ⅰ collagen （ColⅠ）， α-smooth muscle actin （α-SMA）， platelet-derived growth factor receptor-β （PDGFR-β）， NKD2， dishevelled protein 2 （DVL2） and β-catenin in renal tissue were detected by immunohistochemistry and Western blot. ResultsCompared with the normal group， the model group showed significant renal pathological changes， a markedly increased kidney weight/body weight ratio （P<0.01）， significantly elevated CVF （P<0.01）， and notably increased serum levels of SCr， BUN， IL-1β， and IL-6 （P<0.01）. Expression levels of FN1， ColⅠ， α-SMA， PDGFR-β， NKD2， DVL2， and β-catenin in renal tissue were also significantly increased （P<0.01）. Compared with the model group， all treatment groups showed significantly decreased kidney weight/body weight ratios and CVF （P<0.01）， as well as markedly decreased serum SCr， BUN， IL-1β， and IL-6 levels. Protein expression levels of FN1， ColⅠ， α-SMA， PDGFR-β， NKD2， DVL2， and β-catenin in renal tissue were decreased， with more pronounced effects observed in the Niaoduqing， medium-dose， and high-dose Shenshu Fujian decoction groups （P<0.05， P<0.01）. ConclusionShenshu Fujian decoction improves renal function， reduces inflammation， and reverses renal fibrosis in CRF rats， possibly by downregulating the expression of PDGF/NKD2/Wnt signaling pathway-related proteins.
