Effects of Blue Light on Emmetropization in Guinea Pigs Based on Proteomic Analysis
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2025.0212
- VernacularTitle:基于蛋白组学分析蓝光对豚鼠眼球正视化过程的影响
- Author:
Junxin XIAO
1
;
Zhuoya QUAN
1
;
Hu XIAO
2
;
Thomas Cheun LAM
2
;
Minyi ZHU
1
;
Danyang WANG
1
Author Information
1. Department of Ophthalmology， The Seventh Affiliated Hospital， Sun Yat-sen University，Shenzhen 518107， China
2. Shenzhen Research Institute， The Hong Kong Polytechnic University， Shenzhen 518052， China
- Publication Type:Journal Article
- Keywords:
blue light;
emmetropization;
myopia;
refractive development;
proteomics
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2025;46(2):284-292
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of blue light on emmetropization in guinea pigs， explore the potential mechanisms and assess its application in myopia prevention and control. MethodsThree-week-old male guinea pigs （n=20） were randomly assigned to the white light group and the blue light group. Refraction and ocular biological parameters were measured every 2 weeks until the experiment ended at week 8. And the 4D-data-independent acquisition （4D-DIA） proteomics technology was used to analyze retina from both the blue light and white light groups， exploring protein composition， expression differences， and biological functions. ResultsAfter 2 weeks， Guinea pigs exposed to white light gradually tended towards emmetropia， showing a statistically significant difference in refractive error compared to the blue light group （P<0.001）. From week 4， the axial length of the blue light group was significantly shorter than that of the white light group （P<0.05）. Meanwhile， the vitreous chamber length in the blue light group was significantly smaller than that of the white light group from week 2 （P<0.05）. A total of 161 differentially expressed proteins were identified by proteomics technology in the retina， with 98 proteins upregulated and 63 proteins downregulated. These proteins were primarily enriched in biosynthetic pathways such as vesicle transport， redox reaction， niacin and nicotinamide metabolism and NAD+ metabolism. ConclusionsGuinea pigs raised under blue light exhibit hyperopic drift and slowed axial elongation， which slows the procession of emmetropization. Based on the 4D-DIA technology， the differentially expressed proteins between the blue light and white light groups are primarily involved in NAD+ metabolism， niacin and nicotinamide metabolism. Especially in NAD+ salvage synthesis， nicotinamide phosphoribosyl transferase （NAMPT） is upregulated， while sirtuin 2 （SIRT2） is downregulated. It provides new insights into the mechanism of blue light in emmetropization and a theoretical basis for myopia prevention and control.
