Enhancement of Microglial Phagocytosis by Scaffold Protein PDLIM5 and Its Role in Multiple Sclerosis
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2025.0201
- VernacularTitle:支架蛋白PDLIM5增强小胶质细胞吞噬及其在多发性硬化中作用的研究
- Author:
Hailian CHEN
1
;
Yuge WANG
2
;
Yu CUI
1
;
Suning PING
1
;
Yuan CHEN
1
Author Information
1. Sun Yat-sen University School of Medicine， Shenzhen 518107， China
2. Department of Neurology， The Third Affiliated Hospital of Sun Yat-sen University， Guangzhou 510630， China
- Publication Type:Journal Article
- Keywords:
PDLIM5;
multiple sclerosis;
microglia;
phagocytosis;
neuroinflammation
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2025;46(2):179-185
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression of scaffold protein PDLIM5 in multiple sclerosis （MS） patients and the mouse microglial cell line BV2， and to explore its effects on the phagocytosis of microglial cells. MethodsPeripheral blood samples were collected from 24 MS patients and 6 healthy volunteers as controls. The expression levels of PDLIM5 were detected by real-time quantitative PCR. A neuroinflammation cell model was established by treating the mouse microglial cell line BV2 with lipopolysaccharide （LPS， 1 µg/mL）. The expression levels of PDLIM5 were measured by Western Blot. The effect of PDLIM5 expression on phagocytosis was analyzed by transfecting BV2 cells with PDLIM5 shRNA plasmids or PDLIM5 overexpression plasmids. ResultsReal-time quantitative PCR results showed that compared with the healthy control group， the expression level of PDLIM5 from the MS patients was significantly increased in monocytes ［2.78 （0.70-6.86） vs. 0.54 （0.39-1.51）， P=0.036］ and lymphocytes ［1.62 （0.90-2.26） vs. 0.11 （0.05-0.21）， P<0.001］. Western Blot results indicated that PDLIM5 expression was significantly upregulated in BV2 cells following LPS stimulation （P<0.05）. Plasmid transfection experiments demonstrated that knockdown of PDLIM5 inhibited the phagocytic capacity of BV2 cells as measured by trypan blue uptake （P<0.05）， while overexpression of PDLIM5 enhanced the phagocytic ability of BV2 cells （P<0.001）. ConclusionUnder neuroinflammatory conditions， PDLIM5 expression is elevated， and this upregulation promotes the phagocytosis of microglial cell.
