Expression and Clinical Significance of Nucleoporin 93 in Patients with Neuroblastoma
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2025.0307
- VernacularTitle:核孔蛋白93在神经母细胞瘤患者中的表达及临床意义
- Author:
Minting LIANG
1
;
Yang YANG
1
;
Xiaojun LIU
1
;
Huiya LIANG
1
;
Hanyi ZHANG
2
;
Yihan SUN
2
;
Xiuyu SHI
2
;
Xia YANG
1
Author Information
1. Department of Biochemistry and Molecular Biology， Zhongshan School of Medicine， Sun Yat-sen University， Guangzhou 510080， China
2. Zhongshan School of Medicine， Sun Yat-sen University， Guangzhou 510080， China
- Publication Type:Journal Article
- Keywords:
neuroblastoma;
nucleoporin 93;
differentiation;
prognosis;
biomarker
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2025;46(3):420-430
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo screen key genes associated with neuroblastoma （NB） diagnosis and prognosis using the Gene Expression Omnibus （GEO） database， and to investigate the expression and clinical significance of nucleoporin 93 （NUP93） in NB tissues. MethodsNB gene chip data （GSE73517， GSE49710， GSE19274） were retrieved from the GEO database. Differentially expressed genes （DEGs） commonly upregulated in high-risk groups were screened. The R2 database was then used to assess the prognostic value of DEGs that were commonly upregulated in the MYCN amplification group. Finally， NUP93 expression levels in the tissues from 60 NB， 25 ganglioneuroblastoma （GNB）， and 26 ganglioneuroma （GN） cases were measured by immunohistochemistry . ResultsTwenty-five DEGs were identified as commonly upregulated in high-risk groups. Among these， 10 genes （SIVA1， NUP93， STIP1， LSM4， RAI14， MYOZ3， KNTC1， TNFRSF10B， TACC3 and CEP152） showed significantly higher expression in MYCN-amplified subgroups （P＜0.05）. Survival analysis revealed that high NUP93 expression was associated with shorter overall survival （HR = 4.0， 95% CI： 3.0，5.3， P = 1.80 × 10⁻³⁴）. Immunohistochemistry results revealed that NUP93 expression in NB tissues was significantly higher than in GNB and GN tissues （P＜0.001）. NUP93 expression was positively correlated with high mitosis-karyorrhexis index （MKI； P=0.040）， poor differentiation （P＜0.001）， and MYCN expression （rs = 0.793， P ＜0.001）. ConclusionsHigh expression of NUP93 is associated with high MKI and poor differentiation， and predicts unfavorable prognosis in patients with NB， suggesting it may promote tumor progression by regulating MYCN. NUP93 has the potential to be a novel diagnostic biomarker and therapeutic target for NB.
