Improvement effects and mechanism of total secondary ginsenosides on hypertrophic changes in cardiomyocytes
- VernacularTitle:人参总次苷对心肌细胞肥大性改变的改善作用及机制
- Author:
Bin LI
1
,
2
;
Jia LI
1
;
Zhongjie YUAN
1
;
Mingjun ZHU
1
;
Shiyang XIE
1
;
Yuan GAO
1
;
Rui YU
1
;
Xinlu WANG
1
Author Information
1. Heart Center，the First Affiliated Hospital of Henan University of Chinese Medicine，Zhengzhou 450099，China
2. Henan Evidence-based Traditional Chinese Medicine Center，Zhengzhou 450099，China
- Publication Type:Journal Article
- Keywords:
total secondary ginsenosides;
heart failure;
cardiomyocyte;
hypertrophic change;
energy metabolism;
glycolysis
- From:
China Pharmacy
2025;36(12):1430-1435
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the ameliorative effects and potential mechanism of total secondary ginsenosides （TSG） on hypertrophic changes of primary cardiomyocytes stimulated by angiotensin Ⅱ （Ang Ⅱ）. METHODS Primary cardiomyocytes were isolated from the hearts of neonatal SD rats and divided into the following groups： control group， AngⅡ group （2 µmol/L）， TSG group （7.5 µg/mL）， PFK-015 group [6-phosphofructo-2-kinase/fructose-2， 6-bisphosphatase 3 （PFKFB3） inhibitor， 10 nmol/L]， and TSG+PFK-015 group （TSG 7.5 µg/mL+PFK-015 10 nmol/L）. The surface area， protein synthesis， energy metabolism-related indicators [free fatty acid （FFA）， coenzyme A （CoA）， acetyl coenzyme A （acetyl-CoA）]， and the expressions of glycolysis-related factors [hypoxia-inducible factor 1α （HIF-1α）， glucose transporter protein 4 （GLUT-4）， lactate dehydrogenase A （LDHA）， pyruvate dehydrogenase kinase 1 （PDK1） and PFKFB3] in primary cardiomyocytes of each group were measured. RESULTS Compared with the control group， the surface area of primary cardiomyocytes and protein synthesis were significantly increased， the content of FFA， protein and mRNA expressions of HIF-1α， LDHA， PDK1 and PFKFB3 were significantly increased or up-regulated in the AngⅡ group， while the contents of CoA and acetyl-CoA， the protein and mRNA expressions of GLUT-4 were significantly decreased or down-regulated （P＜0.05）. Compared with the AngⅡ group， both TSG group and PFK-015 group showed significant improvements in these indexes， with the TSG+PFK-015 group generally demonstrating superior effects compared to either treatment alone （P＜0.05）. CONCLUSIONS TSG can reduce the surface area of AngⅡ-induced primary cardiomyocytes， decrease protein synthesis， and inhibit their hypertrophic changes. These effects may be related to improving energy metabolism and the inhibition of glycolysis activity.
