Expression and Clinical Significance of PLCβ4 Gene in Hepatocellular Carcinoma Analyzed Based on TCGA Database and Experimental Validation
10.3971/j.issn.1000-8578.2025.24.1044
- VernacularTitle:基于TCGA数据库和实验验证分析肝细胞癌中PLCβ4基因的表达及临床意义
- Author:
Limei WEN
1
,
2
;
Yali GUO
3
,
4
;
Qiang HOU
4
;
Dongxuan ZHENG
4
;
Wu DAI
4
;
Xiang GAO
4
;
Jianhua YANG
5
;
Junping HU
3
,
4
Author Information
1. Department of Pharmacy, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
2. State Key Laboratory of Neurology and Oncology Drug Development, Nanjing 210042, China.
3. State Key Laboratory of Neurology and Oncology Drug Development, Nanjing 210042, China
4. College of Pharmacy, Xinjiang Medical University, Urumqi 830017, China.
5. Department of Pharmacy, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China.
- Publication Type:CLINICALRESEARCH
- Keywords:
Hepatocellular carcinoma;
Phospholipase-Cβ4 gene;
TCGA database;
Phosphatidylinositol signaling genes
- From:
Cancer Research on Prevention and Treatment
2025;52(6):502-510
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the PLCβ4 gene mRNA expression and its clinical significance in hepatocellular carcinoma (HCC) based on TCGA database. Methods Based on the data on 424 clinical samples (including 374 cases of HCC tissues and 50 cases of nontumor liver tissues) in the TCGA database, Kaplan–Meier method, Cox regression analysis, and immune infiltration analysis were performed to evaluate the relationship between PLCβ4 gene and the clinical characteristics and survival prognosis of HCC patients. Correlation analysis between PLCβ4 gene and 24 types of immune cells was applied to investigate the relationship between PLCβ4 gene and immune cell infiltration and mRNA expression level of TP53 gene, a high-frequency mutation gene in HCC. In addition, paraffin sections of highly, moderately, and poorly differentiated tumor tissues and normal liver tissues from HCC patients were collected. The histopathological observation was carried out via HE staining method, and the expression levels of PLCβ4 and Ki-67 proteins in each clinical sample were verified through the immunohistochemical method. Results The expression level of PLCβ4 gene in HCC was significantly higher than that in normal tissues (P<0.01), and all patients in the PLCβ4 high-expression group had a significantly longer overall survival than those in the low-expression group (P<0.05), which suggested that PLCβ4 substantially affected the prognosis of HCC patients. Correlation analysis showed that the expression level of PLCβ4 gene was highly correlated with immune cell infiltration and the expression level of TP53 gene. As verified by clinical sample experiments, HE staining experiments and immunohistochemical results revealed that PLCβ4 gene expression in HCC tissue samples was significantly higher than that in normal tissues (P<0.001), and it was negatively correlated with the degree of differentiation. Conclusion PLCβ4 may serve as an independent prognostic factor in HCC and is expected to be a novel molecular target for HCC treatment.
