Determine antimicrobial preservatives thimerosal in eye drops by HPLC
10.12206/j.issn.2097-2024.202404059
- VernacularTitle:滴眼液中抑菌剂硫柳汞的HPLC含量测定方法研究
- Author:
Jin GAO
1
;
Dan HU
1
;
Zihui MA
1
;
Junwei XU
1
;
Qing GAO
2
;
Xiaoxu HONG
3
Author Information
1. General Station for Drug & Instrument Supervision of Joint Logistics Support Force of the PLA, Beijing 100071, China.
2. Beijing Institute for Drug Control, Beijing 102206, China.
3. Chinese Pharmacopoeia Commission, Beijing 100061, China.
- Publication Type:Researchreports
- Keywords:
HPLC;
eye drop;
antimicrobial preservatives;
thimerosal;
determination
- From:
Journal of Pharmaceutical Practice and Service
2025;43(6):293-297
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determining thimerosal compounds in eye drops. Methods A gradient HPLC system was used in the quantitative analysis of thimerosal compounds on Shiseido MGII C18 column (4.6 mm×250 mm,5 μm), using 1% triethylamine solution (pH adjusted to 3.0 with phosphate) as mobile phase A, the methanol as mobile phase B, gradient elution, The column temperature was 40 ℃, the detection wavelength was 222 nm, the flow rate was 1 ml/min and the injection volume was 20 µl. Results The established method had good linearity within the concentration range of 4.3-216.7 μg/ml (r>0.999) for thimerosal, with average recoveries was 102.1%, RSD2.7%. Conclusion This method was simple, accurate and highly specific, and could be used for determination of thimerosal compound in eye drops.