Regulatory mechanism of HIF-1α/HO-1/VEGF-A signaling pathway in immaturity of autogenous arteriovenous fistula
- VernacularTitle:HIF-1α/HO-1/VEGF-A信号通路在自体动静脉内瘘成熟不良中的调控机制
- Author:
Xiang FANG
1
;
Xiaolu SUI
;
Wentong HUANG
;
Jiahui CHEN
;
Xiaoling ZHONG
;
Jihong CHEN
Author Information
- Publication Type:Research Article
- Keywords: hypoxia-inducible factor-1α; heme oxygenase-1; vascular endothelial growth factor A; signaling pathway; arteriovenous fistula; endothelial progenitor cells
- From: Journal of Clinical Medicine in Practice 2024;28(18):101-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the differences in the expression levels of the hypoxia-inducible factor-1α/heme oxygenase-1/vascular endothelial growth factor A (HIF-1α/HO-1/VEGF-A) signaling pathway under different maturation outcomes of arteriovenous fistula (AVF) in patients with stage 5 chronic kidney disease, and explore the regulatory mechanism of the HIF-1α/HO-1/VEGF-A signaling pathway in the maturation process of AVF. Methods A total of 45 patients with AVF surgery for stage 5 chronic kidney disease were selected as research objects, vascular specimens were collected during the AVF surgery, and the patients were divided into AVF maturation group (
n =35) and AVF poor maturation group (n =10) based on the AVF maturation outcomes within 8 weeks after surgery. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the gene expression levels ofHIF-1α ,HO-1 andVEGF-A in the HIF-1α/HO-1/VEGF-A signaling pathway; the Western blotting was used to detect the protein expression levels of HIF-1α, HO-1 and VEGF-A; the enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of downstream inflammatory factors such as interleukin-1β (IL-1β), interleukin-8 (IL-8), and transforming growth factor-β (TGF-β). Results The expression levels ofHIF-1α mRNA,HO-1 mRNA, andVEGF-A mRNA in the AVF maturation group were significantly higher than those in the AVF poor maturation group (P < 0.01); the protein expression levels of HIF-1α, HO-1 and VEGF-A in the AVF maturation group were significantly higher than those in the AVF poor maturation group (P < 0.01); the expression levels of IL-1β, IL-8 and TGF-β in the AVF maturation group were significantly lower than those in the AVF poor maturation group (P < 0.01). Conclusion The upregulation of the HIF-1α/HO-1/VEGF-A signaling pathway is conducive to the early maturation of AVF. The measurement of the expression levels of the HIF-1α/HO-1/VEGF-A signaling pathway has a certain predictive significance for the early maturation outcome of AVF.
