Circ_0114427 targets microRNA-330-5p to regulate apoptosis and inflammatory response in lipopolysaccharide-induced alveolar epithelial cells
- VernacularTitle:circ_0114427靶向微小RNA-330-5p调控脂多糖诱导的肺泡上皮细胞凋亡及炎症反应
- Author:
Zhongxin YANG
1
;
Ran ZHOU
;
Zhichao HUANG
;
Xiaohan MEI
;
Yijian LI
;
Xuefeng YANG
;
Xiaoyu LI
Author Information
- Publication Type:Research Article
- Keywords: alveolar epithelial cells; circ_0114427; microRNA-330-5p; apoptosis; inflammation
- From: Journal of Clinical Medicine in Practice 2024;28(6):24-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of circ_0114427 on apoptosis and inflammation of alveolar epithelial cells induced by lipopolysaccharide (LPS) and its mechanism. Methods Human alveolar epithelial cells were cultured
in vitro and transfected with si-circ_0114427, microRNA (miR)-330-5p mimics or co-transfected with si-circ_0114427 and anti-miR-330-5p, and the cellswere then treated with 10 mg/L LPS for 24 hours. The expression levels of circ_0114427 and miR-330-5p in the cells were detected using real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). The protein levels of Cleaved-caspase3 and Cleaved-caspase9 were evaluated using western blot. Enzyme-linked immunosorbent assay (ELISA) was used to assess the expression of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Cell apoptosis was analyzed using flow cytometry. The interaction between circ_0114427 and miR-330-5p was validated using a dual luciferase reporter assay. Results After LPS treatment of alveolar epithelial cells, the expression of circ_0114427 was upregulated, while miR-330-5p expression was downregulated in in the cells (P < 0.05). Knockdown of circ_0114427 or upregulation of miR-330-5p could inhibit LPS-induced apoptosis, Cleaved-caspase3, Cleaved-caspase9, IL-6, and TNF-α levels in alveolar epithelial cells (P < 0.05). Circ_0114427 directly interacted with and negatively regulated miR-330-5p. Downregulation of miR-330-5p reversed the inhibitory effect of circ_0114427 knockdown on LPS-induced cell apoptosis and inflammation. Conclusion Knockdown of circ_0114427 can inhibit LPS-induced apoptosis and inflammation in alveolar epithelial cells, possibly through upregulation of miR-330-5p.
