Evaluation of growth-promoting effect of PL-NBCS instead of FBS on BHK-21, Vero and HEK293T cells
10.13200/j.cnki.cjb.004499
- VernacularTitle:含血小板裂解液的新生牛血清替代胎牛血清培养BHK-21、Vero、HEK293T细胞的促生长效果评价
- Author:
WANG Limei
- Publication Type:Journal Article
- Keywords:
Platelet lysate(PL);
Newborn calf serum(NBCS);
Fetal bovine serum(FBS);
Suspension cell culture;
Adherent cell culture
- From:
Chinese Journal of Biologicals
2025;38(06):725-730
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the growth-promoting effects of platelet lysate-containing newborn calf serum(PL-NBCS) and fetal bovine serum(FBS) on cultured BHK-21,Vero,HEK293T cells,and to provide experimental basis for exploring the feasibility of culturing cells with PL-NBCS instead of FBS.Methods BHK21 cells,Vero cells and HEK293T cells were cultured with PL-NBCS and FBS,respectively.The growth-promoting effect of PL-NBCS on cells was detected by kinetic statistical analysis of morphological observation,average cell proliferation density,viability and doubling time of continuously subcultured cells.Results There was no significant difference in morphological characteristics between three kinds of cells cultured in PL-NBCS and those cultured in FBS.Under the two culture conditions,the cells grew in an "S" shape.The average proliferation density,viability and doubling time of BHK-21 cells cultured in PL-NBCS showed no significant difference with the BHK-21 cells cultured in FBS(t=0.734,0.432 and 0.029,P=0.472,0.671 and 0.977,respectively).The average proliferation density and viability of Vero cells cultured in PL-NBCS were higher than those in FBS group,and the average doubling time was significantly higher than that in FBS group(t=2.228,P=0.042).The average proliferation density,viability and doubling time of HEK293T cells cultured in PL-NBCS were significantly higher than those in FBS group(t=2.309,2.848 and 2.682,P=0.033,0.011 and 0.015,respectively).Conclusion PL-NBCS has better growth-promoting effect on cells,and can replace FBS to subculture cells.
