Identification of stem cell characteristics of human diploid cells 2BS
10.13200/j.cnki.cjb.004509
- VernacularTitle:人二倍体细胞2BS干细胞特性的鉴定
- Author:
WANG Yao
- Publication Type:Journal Article
- Keywords:
Human diploid cells 2BS;
Mesenchymal stem cells(MSCs);
Cell surface markers;
Differentiation
- From:
Chinese Journal of Biologicals
2025;38(06):647-651
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the stem cell characteristics of human diploid cells 2BS and evaluate the potential as biological function positive cells for quality detection of stem cells.Methods Flow cytometry was applied to detect the expression levels of stem cell surface markers in 2BS cells.The staining with alizarin red S,oil red O and alcian blue was used to evaluate the ability of osteogenic,adipogenic and chondrogenic differentiation,respectively.Co-culture with lymphocytes was used to observe the effects on lymphocyte proliferation.Results Human diploid cells 2BS expressed the surface markers CD73,CD 105 and CD90 of stem cells,and possessed the potential of osteogenic differentiation and inhibiting lymphocyte proliferation.Conclusion 2BS cells have several properties of stem cells and could be the positive control for surface markers detection of stem cells by flow cytometry.
