Interleukin-6, tumor necrosis factor-alpha, and antioxidant levels in the umbilical venous plasma of preeclampsia.
- Author:
Yoon Ha KIM
1
;
Tae Bok SONG
;
So Jeong PARK
;
Ji No PARK
;
Kwang Pil JEONG
;
Jong Woon KIM
;
Seok Mo KIM
;
Cheol Hong KIM
;
Moon Kyoung CHO
;
Ki Min KIM
;
Sung Yeul YANG
;
Bong Whan AHN
Author Information
1. Department of Obstetrics and Gynecology, Chonnam National University Medical School, Gwangju, Korea. kimyh@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Preeclampsia;
Umbilical venous plasma;
Interleukin-6;
tumor necrotic factor-;
Antioxidant
- MeSH:
alpha-Tocopherol;
Ascorbic Acid;
Chromatography, Liquid;
Female;
gamma-Tocopherol;
Humans;
Immunoassay;
Interleukin-6*;
Lipid Peroxidation;
Placenta;
Plasma*;
Pre-Eclampsia*;
Pregnancy;
Tumor Necrosis Factor-alpha*;
Vitamin A;
Vitamins
- From:Korean Journal of Obstetrics and Gynecology
2007;50(7):982-990
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Our purpose was to investigate Interleukin-6 (IL-6), tumor necrotic factor-alpha (TNF-alpha), lipid peroxide levels, oxygen-radical absorbance capacity (ORAC), and antioxidant levels in umbilical venous blood plasma and to evaluate the roles of them in the pathophysiology of preeclampsia. STUDY DESIGN: Samples of umbilical venous plasma were obtained from 20 normal and 20 preeclamptic women between 33 and 40 weeks gestation. IL-6 and TNF-alpha was assayed by an enzyme-linked immunoassay. Lipid peroxide levels were measured by thiobarbituric acid reaction. The ORAC values were measured by Cao's method. Ascorbic acid, retinol, alpha-tocopherol, and gamma-tocopherol were measured by high performance liquid chromatography. RESULTS: There was no significant differences of IL-6 levels in umbilical venous plasma between women with normal and preeclampsia (2.79+/-0.21 vs. 2.94+/-0.17 ng/ml). TNF-alpha levels in umbilical venous plasma of women with preeclampsia were significantly higher than that of women with preeclampsia (3.04+/-0.01 vs. 1.40+/-0.01 ng/ml, p<0.01). Lipid peroxide levels in umbilical venous plasma of women with preeclampsia were significantly higher than that of women with normal pregnancy (7.32+/-0.09 vs. 5.18+/-0.14, p<0.01). The ORAC values in umbilical venous plasma of women with preeclampsia were significantly lower than that of women with normal pregnancy (12,836.5+/-249.4 vs. 10,490.2+/-276.9 U/ml, p<0.05). Ascorbic acid levels in umbilical venous plasma of women with preeclampsia were significantly lower than those of women with normal pregnancy (320.2+/-48.5 vs. 538.5+/-68.2 nmol/ml, p<0.05). CONCLUSION: The above results in umbilical venous plasma suggest that the imbalance of lipid peroxidation and antioxidant activity in placenta is involved in the pathophysiology of preeclampsia. Increased TNF-alpha in the umbilical venous plasma showed inflammatory reaction in the placenta would be one of the cause of preclampsia. An antioxidant vitamin, ascorbic acid, may act an important antioxidant factor in preeclampsia.
