The Effect of Atorvastatin on the Development of Puromycin Aminonucleoside(PAN)-induced Nephrosis in Rats.
- Author:
Kwang Hae CHOI
1
;
Hyo Seuk CHUNG
;
Yong Hoon PARK
;
Yong Jin KIM
;
Jeong Hee HA
;
Heung Sik KIM
Author Information
1. Department of Pediatrics, College of Medicine, Yeungnam University, Korea. yhpark@medical.yu.ac.kr
- Publication Type:Original Article
- Keywords:
Puromycin;
Atorvastatin;
Glomerulosclerosis;
Hyperlipidemia
- MeSH:
Animals;
Cholesterol;
Humans;
Hyperlipidemias;
Injections, Subcutaneous;
Male;
Nephrectomy;
Nephrosis*;
Oxidoreductases;
Puromycin*;
Rats*;
Rats, Sprague-Dawley;
Urine Specimen Collection
- From:Journal of the Korean Society of Pediatric Nephrology
2003;7(1):9-15
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Several studies have suggested that hyperlipidemia might be a causative factor contributing to the progression of initial glomerular injury through the development of glomerulosclerosis. We examined the potential beneficial effect of atorvastatin - which blocks the rate limiting step of cholesterol synthesis by inhibiting HMG-CoA reductase - in PAN- induced nephrosis. MATERIALS AND METHODS: Glomerulosclerosis was induced in Sprague-Dawley male rats by repeated administration of PAN. Sprague-Dawley male rats were divided into 3 groups:group I(control), group II(PAN 20 mg/kg, subcutaneous injection), group III(PAN 20 mg/kg subcutaneous injection and atorvastatin 50 mg/kg/day per oral). On the 11th week, upon sacrifice of the experimental animals, blood sampling, 24-hr urine collection and nephrectomy were performed. RESULTS: Group III had significantly lower BUN and higher serum albumin(30.9+/-17.2 vs. 17.3+/-2.5 mg/dL; 2.3+/-0.1 vs. 2.5+/-0.2 g/dL, P<0.05) compared with group II. In the lipid profiles, group III was associated with a reduction in total cholesterol and LDL(291+/-173 vs. 167+/-72 mg/dL; 57+/-53 vs. 27+/-12 mg/dL, P>0.05) compared with group II. Atorvastatin administration lowered the glomerular sclerosing index significantly(26.2% vs. 13.3%, P<0.05). CONCLUSION: Puromycin-induced glomerulosclerosis could be ameliorated by the reduction of hyperlipidemia with atorvastatin. This suggests that hyperlipidemia contributes to the pathogenesis of glomerulosclerosis.
