Comparison of growth and pubertal progression in wild type female rats with different bedding types.
10.6065/apem.2015.20.1.53
- Author:
Byung Ho KANG
1
;
Shin Hee KIM
;
Kyung A JUNG
;
So Youn KIM
;
Sung Hoon CHUNG
;
Young Shil PARK
;
Kyung Lim YOON
;
Kye Shik SHIM
Author Information
1. Department of Pediatrics, Kyung Hee University Hospital at Gangdong, Seoul, Korea. ksshim64@gmail.com
- Publication Type:Original Article
- Keywords:
Endocrine disrupting chemicals;
Bedding;
Growth;
Puberty
- MeSH:
Adolescent;
Animals;
Bedding and Linens;
Body Weight;
Crown-Rump Length;
Endocrine Disruptors;
Endocrine System;
Estradiol;
Estrous Cycle;
Estrus;
Female;
Growth Hormone;
Humans;
Polymerase Chain Reaction;
Puberty;
Rats*;
Rats, Sprague-Dawley;
RNA, Messenger;
Rodentia;
Vaginal Smears;
Wood
- From:Annals of Pediatric Endocrinology & Metabolism
2015;20(1):53-58
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Endocrine-disrupting chemicals interfere with the endocrine system and therefore affect growth and pubertal progression. The study aim was to compare the growth and pubertal progression in wild-type female rats with different bedding types. METHODS: Twenty 5-week-old female wild-type Sprague Dawley rats were randomly assigned to two groups with different bedding types: one group received wood shaving bedding, while a second group received corncob bedding. We determined crown-rump length and body weight as anthropometric measurements and assessed the serum growth hormone (GH) and estradiol levels. The gh1 mRNA expression levels were compared using quantitative real time transcription polymerase chain reaction. The estrous cycle was evaluated by vaginal smear. RESULTS: The anthropometric measurements were not significantly different between the two groups. The mean relative expression of the gh1 gene was lower in the corncob bedding group than that in the wood shaving group (P=0.768). Meanwhile serum GH and estradiol were increased in the wood shaving bedding group; however this difference was not statistically significant. The time to first estrus and the length of the estrous cycle were increased in the corncob bedding group; the proportion of normal estrous cycles was also decreased. These findings indicate irregularities in the estrous cycle. CONCLUSION: Endocrine-disrupting chemicals in corncob bedding might be associated with time to first estrus and length of the estrous cycle. Therefore, the type of bedding should be considered as a factor affecting pubertal progression in rodents.
