Cytotoxic T lymphocyte‐associated antigen‐4 (CTLA‑4) gene polymorphismsin a cohort of Egyptian patients with immune thrombocytopenia (ITP)
10.1007/s44313-024-00011-z
- Author:
Doaa Mohamed El DEMERDASH
1
;
Maha Mohamed SABER
;
Alia AYAD
;
Kareeman GOMAA
;
Mohamed Abdelkader MORAD
Author Information
1. Internal Medicine Department, Faculty of Medicine, Teaching Kasr AL-Ainy Hospital, Cairo University, Al Kasr Al Aini, Old Cairo 4240310, Cairo Governorate, Egypt
- Publication Type:RESEARCH
- From:Blood Research
2024;59():8-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Immune thrombocytopenia (ITP) is characterized by immune response dysregulations. Cytotoxic T lym-phocyte‐associated antigen‐4 (CTLA‐4) plays a central role in immune checkpoint pathways and preventing autoim-mune diseases by regulating immune tolerance. We aimed to explore the potential association between CTLA-4 gene polymorphisms and ITP as well as study their impact on the response to therapy.
Methods:We investigated two CTLA-4 single‐nucleotide polymorphisms (SNPs; rs: 231775 and rs: 3087243) usingreal-time PCR as well as the plasma levels of CTLA-4 by ELISA in 88 patients with ITP and 44 healthy participants (HC).
Results:CTLA-4 (rs: 3087243) A > G polymorphism analysis showed most HC had the homozygous AA genotype, which was statistically significant compared to patients with ITP. Plasma levels of CTLA4 were statistically lower in patients with acute ITP. There was no correlation between CTLA-4 (rs: 231775 and rs: 3087243) A/G SNPs were not correlated to the response to all lines of therapy assessed (corticosteroids, thrombopoietin receptor agonists, splenectomy, and rituximab).
Conclusion:CTLA-4 CT 60 A/G may affect the susceptibility of ITP, but both CTLA-4 + 49 A/G and CT60 A/G did not impact the response of patients with ITP to different lines of therapy.
