Optimized clade 2.3.2.1c H5N1 recombinant-vaccine strains against highly pathogenic avian influenza.
10.4142/jvs.2017.18.S1.299
- Author:
Jin Wook JANG
1
;
Chung Young LEE
;
Il hwan KIM
;
Jun Gu CHOI
;
Youn Jeong LEE
;
Seong Su YUK
;
Ji Ho LEE
;
Chang Seon SONG
;
Jae Hong KIM
;
Hyuk Joon KWON
Author Information
1. Laboratory of Avian Diseases, Seoul National University, Seoul 88026, Korea.
- Publication Type:Original Article
- Keywords:
influenza A virus H5N1 subtype;
polymerase basic 2 gene;
reverse genetics;
vaccines;
virulence
- MeSH:
Animals;
Chickens;
Efficiency;
Eggs;
Hemagglutinins;
Influenza in Birds*;
Influenza Vaccines;
Kidney;
Mice;
Neuraminidase;
Ovum;
Poultry;
Reverse Genetics;
Seasons;
Vaccines;
Virulence
- From:Journal of Veterinary Science
2017;18(S1):299-306
- CountryRepublic of Korea
- Language:English
-
Abstract:
A/Puerto Rico/8/34 (PR8)-derived recombinant viruses have been used for seasonal flu vaccines; however, they are insufficient for vaccines against some human-fatal H5N1 highly pathogenic avian influenza (HPAI) viruses (HPAIV) due to low productivity. Additionally, the polymerase basic 2 (PB2) protein, an important mammalian-pathogenicity determinant, of PR8 possesses several mammalian-pathogenic mutations. We previously reported two avian PB2 genes (01310 and 0028) related to efficient replication in embryonated chicken eggs (ECEs) and nonpathogenicity in BALB/c mice. In this study, we generated PR8-derived H5N1 recombinant viruses harboring hemagglutinin (attenuated) and neuraminidase genes of a clade 2.3.2.1c H5N1 HPAIV (K10-483), as well as the 01310 or 0028 PB2 genes, and investigated their replication and immunogenicity. Compared with a control virus harboring six internal PR8 genes (rK10-483), the recombinant viruses possessing the 01310 and 0028 PB2 genes showed significantly higher replication efficiency in ECEs and higher antibody titers in chickens. In contrast to rK10-483, none of the viruses replicated in BALB/c mice, and all showed low titers in Madin-Darby canine kidney cells. Additionally, the recombinant viruses did not induce a neutralization antibody but elicited decreased protective immune responses against K10-483 in mice. Thus, the highly replicative and mammalian nonpathogenic recombinant H5N1 strains might be promising vaccine candidates against HPAI in poultry.
