Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle.
10.4142/jvs.2017.18.S1.333
- Author:
Narae KIM
1
;
Yunho JANG
;
Jin Kyoung KIM
;
Soyoon RYOO
;
Ka Hee KWON
;
Miso KIM
;
Shin Seok KANG
;
Hyeon Seop BYEON
;
Hee Soo LEE
;
Young Hee LIM
;
Jae Myung KIM
Author Information
1. Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang 14089, Korea. kimjm88@korea.kr
- Publication Type:Original Article
- Keywords:
Korea;
Mycobacterium bovis;
cattle;
genomics
- MeSH:
Animals;
Cattle*;
Genome;
Genomics;
Korea;
Molecular Typing;
Mycobacterium bovis*;
Mycobacterium tuberculosis;
Mycobacterium*;
Sequence Analysis;
Tandem Repeat Sequences;
Virulence
- From:Journal of Veterinary Science
2017;18(S1):333-341
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis of Mycobacterium (M.) bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined by single-molecule real-time technology, which showed a genome of 4351712 bp in size with a 65.64% G + C content and 4358 protein-coding genes. Comparative genomic analysis with the genomes of Mycobacterium tuberculosis complex strains revealed that all genomes are similar in size and G + C content. Phylogenetic analysis revealed all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. A sequence comparison based on BLASTP analysis showed that M. bovis AF2122/97 was the strain with the greatest number of completely matched proteins to M. bovis 1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits among M. bovis isolates in Korea.
