Potential biomarkers as an indicator of vertical transmission of Johne's disease in a Korean native cattle farm.
10.4142/jvs.2017.18.S1.343
- Author:
Hong Tae PARK
1
;
Hyun Eui PARK
;
Yong Il CHO
;
Eui Hyung KIM
;
Myunghwan JUNG
;
Seung Won SHIN
;
Su Hyung LEE
;
Dae Yong KIM
;
Han Sang YOO
Author Information
1. Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. yoohs@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Korean native cattle;
Mycobacterium avium subsp. paratuberculosis;
biomarker;
subclinical infection;
vertical transmission
- MeSH:
Agriculture*;
Animals;
Antibodies;
Asymptomatic Infections;
Biomarkers*;
Breast Feeding;
Cattle*;
Colostrum;
Feces;
Humans;
Mothers;
Mycobacterium avium subsp. paratuberculosis;
Paratuberculosis*;
Parturition;
Polymerase Chain Reaction;
Transcriptome
- From:Journal of Veterinary Science
2017;18(S1):343-349
- CountryRepublic of Korea
- Language:English
-
Abstract:
Paratuberculosis (PTB) is caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is one of the most widespread and economically important diseases in cattle. After birth, calves are raised with natural breast feeding without separation from their mothers in most Korean native cattle (Hanwoo breed) farms. Vertical transmission of PTB has been reported, but the exact PTB infection route has not been revealed in Hanwoo farms. Calves of MAP seropositive dams were tested for MAP presence and MAP antibodies in feces and tissues. MAP was detected in calf tissues by using polymerase chain reaction. Expressions of genes reported to be prognostic biomarkers of MAP infection changed in both calves and cows (p < 0.05). Expression of two genes (HGF and SERPINE1) were significantly decreased in MAP-infected cattle and their offspring (p < 0.01). The results suggest that biomarker gene expression profiles can be useful in detecting early stage MAP infection. Based on the results, complete eradication of MAP may be possible if accurate diagnostic methods to detect infected calves are added to the current PTB eradication strategy, which, because infected individuals are likely to develop into fecal MAP shedders at any time, includes isolation of new born calves and feeding sterilized colostrum.
