Generation, characterization, and application in serodiagnosis of recombinant swine vesicular disease virus-like particles.
10.4142/jvs.2017.18.S1.361
- Author:
Wanhong XU
1
;
Melissa GOOLIA
;
Tim SALO
;
Zhidong ZHANG
;
Ming YANG
Author Information
1. National Centre for Foreign Animal Disease, Winnipeg, MB R3E 3M4, Canada. Ming.yang@inspection.gc.ca
- Publication Type:Original Article
- Keywords:
enzyme-linked immunosorbent assay;
serological tests;
swine vesicular disease virus;
virus-like particles
- MeSH:
Animals;
Antibodies;
Baculoviridae;
Blotting, Western;
Certification;
Enterovirus B, Human;
Enzyme-Linked Immunosorbent Assay;
Foot-and-Mouth Disease;
Mass Spectrometry;
Methods;
Microscopy, Electron;
Neutralization Tests;
Serologic Tests*;
Swine Vesicular Disease*;
Swine*;
Virus Diseases
- From:Journal of Veterinary Science
2017;18(S1):361-370
- CountryRepublic of Korea
- Language:English
-
Abstract:
Swine vesicular disease (SVD) is a highly contagious viral disease that causes vesicular disease in pigs. The importance of the disease is due to its indistinguishable clinical signs from those of foot-and-mouth disease, which prevents international trade of swine and related products. SVD-specific antibody detection via an enzyme-linked immunosorbent assay (ELISA) is the most versatile and commonly used method for SVD surveillance and export certification. Inactivated SVD virus is the commonly used antigen in SVD-related ELISA. A recombinant SVD virus-like particle (VLP) was generated by using a Bac-to-Bac baculovirus expression system. Results of SVD-VLP analyses from electron microscopy, western blotting, immunofluorescent assay, and mass spectrometry showed that the recombinant SVD-VLP morphologically resemble authentic SVD viruses. The SVD-VLP was evaluated as a replacement for inactivated whole SVD virus in competitive and isotype-specific ELISAs for the detection of antibodies against SVD virus. The recombinant SVD-VLP assay produced results similar to those from inactivated whole virus antigen ELISA. The VLP-based ELISA results were comparable to those from the virus neutralization test for antibody detection in pigs experimentally inoculated with SVD virus. Use of the recombinant SVD-VLP is a safe and valuable alternative to using SVD virus antigen in diagnostic assays.
