Screening of initial processing methods for Ligusticum sinense slice based on differential metabolites
- VernacularTitle:基于差异代谢物筛选茶芎片的初加工方式
- Author:
Yu HE
1
;
Yanjing DONG
1
;
Qian QIN
1
;
Danyang WU
1
;
Conglong XU
2
;
Shouwen ZHANG
1
Author Information
1. Research Center for Chinese Medicinal Materials Resources and Ethnic Medicine，Jiangxi University of Chinese Medicine，Nanchang 330004，China
2. Jiangxi Jingde Traditional Chinese Medicine Co.，Ltd.，Jiangxi Jingdezhen 333302，China
- Publication Type:Journal Article
- Keywords:
Ligusticum sinense;
untargeted metabolomics
- From:
China Pharmacy
2025;36(11):1317-1322
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To screen the primary processing methods of Ligusticum sinense slice based on differential metabolites， and provide theoretical basis for the scientific processing of L. sinense. METHODS Using 13 groups of L. sinense slice processed by fresh-cutting or traditional methods as samples， UHPLC-QE-MS was employed for metabolite identification. Multivariate statistical analysis was applied to screen differential metabolites among the 13 sample groups， analyzing the effects of washing， soaking， drying methods， and drying cycles on both the relative expressions of differential metabolites and the contents of carboxylic acids and their derivatives in the samples （to reflect the total amino acid content）. RESULTS Principal component analysis and partial least squares-discriminant analysis both showed significant intergroup differences among the 13 sample groups. A total of 688 differential metabolites were screened from the 13 sample groups， with carboxylic acids and their derivatives showing the highest proportion. The relative expression levels of phosphatidylcholine significantly increased after washing treatment， while tryptophan expression significantly decreased after soaking treatment. Samples dried at 50-60 ℃ showed significantly increased expression of psoralen， whereas those dried at 40 ℃ showed significantly decreased expression of methyl -p- methoxycinnamate. Both washing and soaking treatments significantly reduced the total amino acid content in samples， while secondary drying significantly increased it. The three controlled-temperature drying methods maintained relatively stable total content of amino acids in samples. CONCLUSIONS The optimal processing protocol for L. sinense slice is as follows: fresh L. sinense slice should be freshly cut at the production site， undergo quick washing after soil removal， and be dried twice at 40 ℃ （before and after slicing）.
