Electrophoretic Analysis of the Major Polypoptides of Human and Rat Skin.
- Author:
Johng Bong KAHNG
- Publication Type:Original Article
- MeSH:
Animals;
Bony Callus;
Circumcision, Male;
Dermis;
Electrophoresis;
Epidermis;
Female;
Gels;
Hand;
Humans*;
Male;
Molecular Weight;
Peptides;
Rats*;
Skin*;
Sodium;
Trypsin
- From:Korean Journal of Dermatology
1979;17(1):1-16
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was designed to analyze the major proteins of skin tissue. Whole skin samples were obtained from rats and from healthy persons by circumcision. Stratum corneum was taken from human callus lesions. Epidermis was separated from dermis by cold trypsinization, and proteins of each tissue were extracted with Trisurea-mercaptoethanol without any elimination of soluble proteins. S-Carboxymethy- lated (SCM)-fibrous protem was partially purified from the stratum corneum, The prepared proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Approximate molecular weights of each polypeptide were determined on polyacrylamide gels, and protein patterns of human and rat tissues were compared. The SCM-fibrous protein of stratum corneum was about 0. 762 g/g tissue. Electrophoresis of the protein revealed faur major polypeptides with molecular weights of 47, 000, 56, 000, 64, 000 and 70, 000. Of these, the 70, 000 Qalton band was the most prominent. Electrophoretic patterns of epidermal proteins were very similar between human and rat tissues, although some minor differences were found. Each preparation showed eleven bands. The bands of 61, 000 dalton and of 62, 000 dalton were the most distinct ones in human and rat epidermis, respectively. The four major lmnda of SCM-fibrous protein purified from stratum corneum showed a similar migration profile to the four pmminent bands found in both epidermal preparations. Derrnal proteins, however, had no similarity between human and rat tissues. Irx hman dermis, there were nine major electrophoretic bands with similar density, which located mainly in the center of the gel. In the rat dermal preparation, electroporetic analysis showed ten major bands and most of these polypeptides had high molecular weights with five bands being metachromatic. Furthermore, a periedic acid-Schiff-positive hand was found only in rat dermal tissue. These results suggest that the major, epidermal proteins are similar between. human and rat skin, and that fibrous protein is the major protein in the epidermis of both tissues; however, that dermal proteins are different between human and rat skin.
