Evaluation of a Simple Immunochromatographic Assay as Qualitative Screening Test for Anti-PGL-I Antibodies in Serodiagnosis of Leprosy (I).
- Author:
ong Pill KIM
1
;
Sang Nae CHO
;
Young Hoon KO
Author Information
1. Institute for Leprosy Research, Korean Hansen Welfare Association Department of Microbiology, Yonsei University College of Medicine, Korea. dr_jpkim@hotmail.com
- Publication Type:Original Article
- Keywords:
PGL-I;
immunochromatographic assay
- MeSH:
Antibodies*;
Enzyme-Linked Immunosorbent Assay;
Humans;
Immunochromatography*;
Leprosy*;
Mass Screening*;
Mycobacterium leprae;
Phenol;
Sensitivity and Specificity;
Seroepidemiologic Studies;
Serologic Tests*
- From:Korean Leprosy Bulletin
2004;37(2):10-18
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Among many reported applications of detection of antibodies to phenolic glycolipid-I (PGL-I) of Mycobacterium leprae, seroprevalence may turn out to be very useful as an indicator of the magnitude of leprosy problem in its control program. Recently, simple tests for the detection of antibodies to PGL-I have been developed. As an effort to assess meaning of qualitative test for anti-PGL-I antibodies, we compared enzyme-linked immunosorbent assay (ELISA) and an immunochromatographic assay (ICA) using PGL-I neoglycoconjugate antigens for detection of antibodies in sera from 165 leprosy patients and 746 healthy controls. The upper limit of normal control O.D. in ELISA was 0.236, and the sensitivity of ICA was 56.4%, and its specificity was 93.9%.
