Purification of G6PD from erythrocytes by affinity chromatography
- Author:
Phuong Thi Vu
- Publication Type:Journal Article
- Keywords:
erythrocyte
- MeSH:
Isolation & purification;
Erythrocytes;
Chromatography
- From:Journal of Vietnamese Medicine
1998;230(11):8-12
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
The procedure using affinity chromatography for purification of G6PD from rabbit erythrocytes has been developed. This method consists of three following steps: ion exchange chromatography on DEAE - Sephadex A50, precipitation with ammonium sulfate and affinity chromatography on 2'5' ADP - Sepharose 4B. The first and the second step are directly derived from the procedure of NguyÔn H÷u ChÊn. By our method, the accumulative purification of G6PD from rabbit erythrocytes was 10243 fold. A specific activity of 69.66 IU/mg protein and a final yield of 40% were obtained.
